+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The effect of stress on the expression of GnRH and GnRH receptor genes in the discrete regions of the hypothalamus and pituitary of anestrous ewes



The effect of stress on the expression of GnRH and GnRH receptor genes in the discrete regions of the hypothalamus and pituitary of anestrous ewes



Reproductive Biology 7(1): 55-71



Using the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique, the gonadotropin releasing-hormone (GnRH) mRNA and GnRH receptor (GnRH-R) mRNA were analyzed in the preoptic area (POA), anterior (AH) and ventromedial (VM) hypothalamus, stalk/median eminence (SME) and anterior pituitary gland (AP) of anestrous ewes subjected to short or prolonged footshock stimulation. No GnRH gene expression was detected in the SME and AP. The comparable levels of GnRH mRNA were found in the POA, AH and VM in control ewes. Short and prolonged footshock stimulation significantly increased GnRH mRNA in all analyzed tissue. The highest responses in GnRH mRNA to the short stress occurred in the POA whereas to the prolonged stress in the POA and VM. In non-stressed ewes the GnRH-R mRNA were detected in tissue continuum throughout the POA, AH, VM, SME and AP. The highest concentration of GnRH-R mRNA was detected in the SME. Short as well as prolonged stress stimuli caused an increase in GnRH-R mRNA levels in all analyzed tissue. The highest responses in GnRH-R mRNA expression were found in the VM. In spite of profound up-regulation of GnRH mRNA and GnRH-R mRNA under the short and prolonged stress conditions, the increase of luteinizing hormone (LH) secretion was noted only during acute stress. It is suggested that the increase of expression of GnRH and GnRH-R genes in anestrous ewes are not directly related to GnRH level and GnRH-R activity.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 056311809

Download citation: RISBibTeXText

PMID: 17435833


Related references

Expression of the GnRH and GnRH receptor (GnRH-R) genes in the hypothalamus and of the GnRH-R gene in the anterior pituitary gland of anestrous and luteal phase ewes. Animal Reproduction Science 108(3-4): 345-355, 2008

Expression of the GnRH and GnRH receptor genes in the hypothalamus and of the GnRH-R gene in the anterior pituitary gland of anestrous and luteal phase ewes. Animal Reproduction Science8: 3 4, 345-355, 2008

Effect of stress on the expression of GnRH and GnRH receptor (GnRH-R) genes in the preoptic area-hypothalamus and GnRH-R gene in the stalk/median eminence and anterior pituitary gland in ewes during follicular phase of the estrous cycle. Acta Neurobiologiae Experimentalis 67(1): 1-12, 2007

Implication of dopaminergic systems on GnRH and GnRHR genes expression in the hypothalamus and GnRH-R gene expression in the anterior pituitary gland of anestrous ewes. Experimental and Clinical Endocrinology and Diabetes 116(6): 357-362, 2008

Effect of endotoxin on the expression of GnRH and GnRHR genes in the hypothalamus and anterior pituitary gland of anestrous ewes. Animal Reproduction Science 120(1-4): 105-111, 2010

The central effect of beta-endorphin and naloxone on the expression of GnRH Gene and GnRH receptor (GnRH-R) gene in the hypothalamus, and on GnRH-R gene in the anterior pituitary gland in follicular phase ewes. Experimental and Clinical Endocrinology and Diabetes 116(1): 40-46, 2008

Changes in the GnRH mRNA and GnRH receptor (GnRH-R) mRNA levels in the hypothalamic-anterior pituitary unit of anestrous ewes after infusion of GnRH into the third cerebral ventricle. Reproductive Biology 8(2): 149-161, 2008

Effects of GABA(A) receptor modulation on the expression of GnRH gene and GnRH receptor (GnRH-R) gene in the hypothalamus and GnRH-R gene in the anterior pituitary gland of follicular-phase ewes. Animal Reproduction Science 111(2-4): 235-248, 2009

Neuroendocrine regulation of GnRH release and expression of GnRH and GnRH receptor genes in the hypothalamus-pituitary unit in different physiological states. Reproductive Biology 10(2): 85, 2010

Effect of short-term and prolonged stress on the biosynthesis of gonadotropin-releasing hormone (GnRH) and GnRH receptor (GnRHR) in the hypothalamus and GnRHR in the pituitary of ewes during various physiological states. Animal Reproduction Science 174: 65-72, 2016

Involvement of gamma amino butyric acid (GABA) in the postnatal function of the GnRH pulse generator as determined on the basis of GnRH and GnRH-receptor gene expression in the hypothalamus and the pituitary. Experimental and Clinical Endocrinology and Diabetes 105(6): 353-358, 1997

Effects of corticotropin-releasing hormone and its antagonist on the gene expression of gonadotrophin-releasing hormone (GnRH) and GnRH receptor in the hypothalamus and anterior pituitary gland of follicular phase ewes. Reproduction Fertility and Development 23(6): 780-787, 2011

Effect of GnRH pulse frequency on pituitary concentrations of LH and FSH, GnRH receptors , gonadotropin subunit mRNA, and GnRH-R mRNA in anestrous cows. Journal of Animal Science 74(Suppl. 1): 231, 1996

Estradiol and gonadotropin-releasing hormone (GnRH) interact to increase GnRH receptor expression in ovariectomized ewes after hypothalamic-pituitary disconnection. Endocrine 8(3): 225-229, 1998

The Central Effect of β-Endorphin and Naloxone on The Biosynthesis of GnRH and GnRH Receptor (GnRHR) in The Hypothalamic-Pituitary Unit of Follicular-Phase Ewes. Reproduction in Domestic Animals 51(4): 555-561, 2016