Section 57
Chapter 56,317

The effects and mechanisms of Forsythia suspense on the expression of Foxp3 on splenocytes and level of Treg in peripheral blood in severely burnt rats

Fu, Y.-J.; Yuan, J.-L.; Chen, J.; Xie, Y.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 25(10): 935-937


ISSN/ISBN: 1007-8738
PMID: 19811747
Accession: 056316539

To explore the immunity modulation function of aqueous of Forsythia suspense (AFS) and its possible mechanisms. Rats of burned model group were burned with vapor under 3 mpa pressure and 108 degrees C temperature for 8 seconds to achieve deep partial-thickness burn, to make a thirty percent total body surface area (TBSA) burn. The experiment were divided into five groups: without any treatment; 8PBH group: 8 h after burn; the rats of AFS1 group, AFS2 group and AFS3 group of them were given AFS 5 g/kg, 2.5 g/kg, 1.25 g/kg once a day by Po. pathway for seven days before burns, respectively. Rats were sacrificed before and 8h after burn, The percentage of Treg cells in CD4(+) T cells was detected by flow cytometry (FCM); the expression of Foxp3 mRNA on splenocytes were measured by RT-PCR, and the protein of Foxp3 activity was evaluated by immunohistochemistry staining. Compared with CONTROL GROUP, the expression of Foxp3 mRNA and protein on the splenocytes were upregulated markedly (P<0.01), and the percentage of Treg were significantly increased (P<0.01) in the 8PBH group. AFS1, AFS2 and AFS3 significantly attenuated these increases (P<0.01), which was dose-dependent. AFS has immunity modulation function and mechanism of it is corrected with Foxp3 mRNA on splenocytes.

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