+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

The estimation error of skeletal muscle capillary supply is significantly reduced by 3D method



The estimation error of skeletal muscle capillary supply is significantly reduced by 3D method



Microvascular Research 79(1): 40-46



Capillary supply of individual skeletal muscle fibers is usually evaluated from two-dimensional (2D) images of thin transverse sections by the number of capillary profiles around a fiber (CAF). This method is inherently inaccurate and the resulting capillary length measurement errors can be avoided by using an alternative three-dimensional (3D) approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. We quantified the error of the 2D method and its reduction by using a 3D approach in realistic geometrical models of muscle fiber capillary bed and in true muscle samples. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed. Thus, 3D approach is more sensitive in agreement with the analysis of geometrical models. We conclude that the 3D method, though technically more demanding than 2D method, represents a more precise approach to evaluation of muscle capillarization. Moreover, the 3D method could be applied to other organs and we suggest potential medical applications.

(PDF emailed within 0-6 h: $19.90)

Accession: 056333619

Download citation: RISBibTeXText

PMID: 19913564

DOI: 10.1016/j.mvr.2009.11.005


Related references

Reduced oxidative phosphorylation and proton efflux suggest reduced capillary blood supply in skeletal muscle of patients with dermatomyositis and polymyositis: a quantitative 31P-magnetic resonance spectroscopy and MRI study. Brain 125(Pt 7): 1635-1645, 2002

Reduced fiber size, capillary supply and mitochondrial activity in constitutional thinness' skeletal muscle. Acta Physiologica: E13097-E13097, 2018

Estimation of isogravimetric capillary pressure by a filtration method in skeletal muscle and lung. Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology 57(6): 1817-1823, 1984

Muscle dysfunction during exercise of a single skeletal muscle in rats with congestive heart failure is not associated with reduced muscle blood supply. Acta Physiologica Scandinavica 181(2): 173-181, 2004

Reduced capillary hydraulic conductivity in skeletal muscle and skin in Type I diabetes: a possible cause for reduced transcapillary fluid absorption during hypovolaemia. Diabetologia 43(9): 1178-1184, 2000

The capillary supply of skeletal muscle. Canadian Journal of Sport Sciences 14(3): 130-131, 1989

Capillary supply in regenerated skeletal muscle. Medicine & Science in Sports & Exercise 24(5 SUPPL): S168, 1992

Quantifying the capillary supply of skeletal muscle. Canadian Journal of Sport Sciences 15(2): 84-85, 1990

Capillary supply in hypertrophied human skeletal muscle. Acta Physiologica Scandinavica 114(4): 635-637, 1982

Capillary supply and lipoprotein-lipase activity in skeletal muscle in man. Acta Physiologica Scandinavica 111(3): 383-384, 1981

Adaptability of the anatomical capillary supply to skeletal muscle of fishes. Journal of Zoology (London) 226(4): 691-698, 1992

Skeletal muscle capillary supply in a fish that lacks respiratory pigments. Respiration Physiology 57(2): 201-212, 1984

The cyto architecture and capillary supply in the skeletal muscle of growing dogs. Journal of Anatomy 132(3): 341-356, 1981

Capillary supply of skeletal muscle fibers in untrained and endurance-trained men. American Journal of Physiology 232(6): H705-H712, 1977

Capillary supply in heavy-resistance trained non-postural human skeletal muscle. Acta Physiologica Scandinavica 117(1): 153-155, 1983