+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The value of epididymal protease inhibitor in differential diagnosis between obstructive azoospermia and non-obstructive azoospermia



The value of epididymal protease inhibitor in differential diagnosis between obstructive azoospermia and non-obstructive azoospermia



Andrologia 43(5): 346-352



There are no efficient and noninvasive clinical tests to distinguish between obstructive azoospermia (OA) and non-obstructive azoospermia (NOA). Epididymal protease inhibitor (Eppin) protein is secreted specifically by testes and epididymides in male reproductive system. It does not exist in seminal plasma of patients with OA in theory. The seminal plasma from 40 normal men and 46 azoospermic patients was detected via Western blot for investigating the presence and characteristics of Eppin protein to distinguish between OA and NOA. The cases were diagnosed as NOA whether Eppin in seminal plasma was positive via Western blot analysis. The cases were diagnosed as OA when samples were Eppin-negative. Additionally, percutaneous epididymal sperm aspiration (PESA) and percutaneous testicular sperm aspiration (PTSA) were performed on these patients at the same time as the diagnostic criteria to compare with Western blot analysis. Eppin detection in seminal plasma showed similar effectivity with PESA/PTSA in differential diagnosis between OA and NOA. Compared with PESA/PTSA, Eppin detection is a new, efficient and noninvasive method which has good clinical application.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056532694

Download citation: RISBibTeXText

PMID: 21951200

DOI: 10.1111/j.1439-0272.2011.01085.x


Related references

Differential diagnosis of azoospermia and etiologic classification of obstructive azoospermia: role of scrotal and transrectal US. Radiology 256(2): 493-503, 2010

Reproductive outcomes following surgical sperm retrieval in couples with obstructive azoospermia (OA), non-obstructive azoospermia (NOA) and repeated in vitro fertilization (IVF) failure. Fertility and Sterility 106(3): E225-E226, 2016

Sertoli cells from non-obstructive azoospermia and obstructive azoospermia patients show distinct morphology, Raman spectrum and biochemical phenotype. Human Reproduction 28(7): 1863-1873, 2014

Distinguishing non-obstructive azoospermia from obstructive azoospermia in Taiwanese patients by hormone profile and testis size. Journal of the Chinese Medical Association 81(6): 531-535, 2017

Relevance of testicular histopathology on prediction of sperm retrieval rates after a conventional bilateral multiple Tese in case of non-obstructive azoospermia (Noa) and obstructive-azoospermia (Oa). The Journal of Sexual Medicine 15(7): S283-S284, 2018

Diagnosis and treatment of epididymal obstructive azoospermia by microsurgery. Zhonghua Nan Ke Xue 18(7): 611-614, 2013

Alpha-glycosidase helps the diagnosis of epididymal obstructive azoospermia. Zhonghua Nan Ke Xue 13(1): 30-32, 2007

Expression of DAZ (deleted in azoospermia), DAZL1 (DAZ-like) and protamine-2 in testis and its application for diagnosis of spermatogenesis in non-obstructive azoospermia. Molecular Human Reproduction 4(9): 827-834, 1998

Testicular and epididymal percutaneous sperm aspiration in men with either obstructive or non-obstructive azoospermia. Journal of Urology 163(4 Suppl ): 304, 2000

Intracytoplasmic sperm injection using surgically retrieved epididymal and testicular spermatozoa in cases of obstructive and non-obstructive azoospermia. International Journal Of Andrology. 20(1): 37-44, 1997

Comparison of ART outcome between non-obstructive azoospermia and obstructive azoospermia: 15 years experiences. Fertility and Sterility 102(3): E68-E69, 2014

Epididymal sperm aspiration for obstructive azoospermia. Hinyokika Kiyo. Acta Urologica Japonica 46(8): 581-586, 2000

Microsurgical reconstruction of pre-epididymal obstructive azoospermia. Journal of Urology 163(4 Suppl ): 258, 2000

Azoospermia factor microdeletion in infertile men with idiopathic severe oligozoospermia or non-obstructive azoospermia. African Journal of Urology 21(4): 246-253, 2015

Endocrine studies of azoospermia. II. Serum steroid levels in obstructive azoospermia. Archives of Andrology 3(2): 163-166, 1979