+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Therapy with anti-flagellin A monoclonal antibody limits Pseudomonas aeruginosa invasiveness in a mouse burn wound sepsis model



Therapy with anti-flagellin A monoclonal antibody limits Pseudomonas aeruginosa invasiveness in a mouse burn wound sepsis model



Burns 35(3): 390-396



The aim of this study was to evaluate the effect of an anti-flagellin sub-type monoclonal antibody (anti-fla-a) on Pseudomonas aeruginosa infection in a mouse burn model and to assay bacterial dissemination and invasiveness. After immediate post-burn infection with P. aeruginosa, mortality and morbidity (daily weight changes) were monitored in mice treated with anti-fla-a as compared to untreated mice. Bacterial dissemination and invasiveness were monitored by bacterial counts at the burn site and spleen. Three different timing regimens for anti-fla-a treatment were studied: (a) prophylaxis (pre-infection), (b) therapeutic (post-infection), and (c) combined mode. Combined regimen of anti-fla-a markedly improved survival of mice infected with P. aeruginosa from 6% to 96% (p<0.0001), similar to treatment with Imipenem. Furthermore, a significant improvement in survival was obtained when anti-fla-a was given prior to (75% survival) or post-infection (50% survival). It reduced bacterial load in the spleen (p=0.01), preventing bacterial sepsis. Anti-fla-a is effective in reducing mortality and morbidity in murine P. aeruginosa-infected burn model.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056551511

Download citation: RISBibTeXText

PMID: 18951715

DOI: 10.1016/j.burns.2008.08.014


Related references

Protection studies in a mouse burn wound sepsis model with a human anti pseudomonas aeruginosa flagella monoclonal antibody and its fab 2 and fab fragments. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 123, 1990

Passive immunisation against Pseudomonas aeruginosa recombinant flagellin in an experimental model of burn wound sepsis. Burns 37(5): 865-872, 2011

Flagellin and pilin immunization against multi-drug resistant Pseudomonas aeruginosa protects mice in the burn wound sepsis model. Immunology Letters 176: 8, 2016

Anti flagellin B antibodies in Pseudomonas aeruginosa murine infected burn wound model. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 43: 66, 2003

Immunoprophylaxis of pseudomonas aeruginosa burn wound sepsis with a cross reactive human monoclonal antibody. Abstracts of the Annual Meeting of the American Society for Microbiology 88: 126, 1988

Negative pressure wound therapy decreases mortality in a murine model of burn-wound sepsis involving Pseudomonas aeruginosa infection. Plos one 9(2): E90494, 2014

Phage therapy of Pseudomonas aeruginosa infection in a mouse burn wound model. Antimicrobial Agents and ChemoTherapy 51(6): 1934-1938, 2007

Protective efficacy of Pseudomonas aeruginosa type-A flagellin in the murine burn wound model of infection. Apmis 122(2): 115-127, 2014

Efficacy of antibodies against the N-terminal of Pseudomonas aeruginosa flagellin for treating infections in a murine burn wound model. Plastic and Reconstructive Surgery 117(7): 2284-2291, 2006

Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model. Microbial Pathogenesis 123: 426-432, 2018

The establishment of a Pseudomonas aeruginosa-infected burn-wound sepsis model and the effect of imipenem treatment. Annals of Plastic Surgery 56(6): 674-679, 2006

Cloning, Expression, and Purification of Pseudomonas aeruginosa Flagellin, and Characterization of the Elicited Anti-Flagellin Antibody. Iranian Red Crescent Medical Journal 18(6): E28271, 2016

Silver sulfadiazine anti infect burn sepsis pseudomonas aeruginosa mouse human. Modern Treatment 4(6): 1259-1262, 1967

Importance of flagellin structure in the pathogenesis of Pseudomonas aeruginosa burn wound infections. Abstracts of the General Meeting of the American Society for Microbiology 103: B-286, 2003

Role of motility and flagellin glycosylation in the pathogenesis of Pseudomonas aeruginosa burn wound infections. Infection and Immunity 73(7): 4395-4398, 2005