EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae


Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae



Fems Yeast Research 14(3): 399-411



ISSN/ISBN: 1567-1356

PMID: 24447461

DOI: 10.1111/1567-1364.12138

Yeast has the potential to be used in bulk-scale fermentative production of fuels and chemicals due to its tolerance for low pH and robustness for autolysis. However, expression of multiple external genes in one host yeast strain is considerably labor-intensive due to the lack of polycistronic transcription. To promote the metabolic engineering of yeast, we generated systematic and convenient genetic engineering tools to express multiple genes in Saccharomyces cerevisiae. We constructed a series of multi-copy and integration vector sets for concurrently expressing two or three genes in S. cerevisiae by embedding three classical promoters. The comparative expression capabilities of the constructed vectors were monitored with green fluorescent protein, and the concurrent expression of genes was monitored with three different fluorescent proteins. Our multiple gene expression tool will be helpful to the advanced construction of genetically engineered yeast strains in a variety of research fields other than metabolic engineering.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056566969

Download citation: RISBibTeXText

Related references

A vector for construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae. Plasmid 17(2): 171-172, 1987

A study on expression of mammalian g protein gene in saccharomyces cerevisiae construction of chimeric genes of rat gs alpha and yeast scg1 for expression in saccharomyces cerevisiae. Korean Journal of Biochemistry 20(1): 9-18, 1988

Screening and Genetic Network Analysis of Genes Involved in Freezing and Thawing Resistance in DaMDHAR -Expressing Saccharomyces cerevisiae Using Gene Expression Profiling. Genes 12(2), 2021

Use of a complementary dna expression cloning vector and a secretion vector for mammalian gene expression in saccharomyces cerevisiae. Barr, P J , A J Brake And P Valenzuela (Ed ) Biotechnology Series, 13 Yeast Genetic Engineering Xviii+354p Butterworths: Stoneham, Massachusetts, Usa; London, England, Uk Illus 281-304, 1989

Use of a cDNA expression-cloning vector and a secretion vector for mammalian gene expression in Saccharomyces cerevisiae. Biotechnology 13: 281-304, 1989

Cloning and expression on a multicopy vector of five invertase genes of Saccharomyces cerevisiae. Current Genetics 11(3): 217-225, 1986

Expression of Escherichia coli recA and ada genes in Saccharomyces cerevisiae using a vector with geneticin resistance. Folia Microbiologica 40(3): 257-262, 1995

Saccharomyces cerevisiae glutaredoxin 5-deficient cells subjected to continuous oxidizing conditions are affected in the expression of specific sets of genes. Journal of Biological Chemistry 279(13): 12386-12395, 2004

Cloning and expression of β-glucosidase genes in Escherichia coli and Saccharomyces cerevisiae using shuttle vector pYES 2.0. Smythe 43(2): 129-135, 1998

Cloning and expression of beta-glucosidase genes in Escherichia coli and Saccharomyces cerevisiae using shuttle vector pYES 2.0. Folia Microbiologica 43(2): 129-135, 1998

The expression in saccharomyces cerevisiae of bacterial beta lactamase and other antibiotic resistance genes integrated in a 2 millimicron dna vector. Cummings, D J Et Al (Ed ) Icn-University Of California, Los-Angeles Symposia on Molecular And Cellular Biology, Vol 15 Extrachromosomal Dna; Proceedings Of The Symposia, Keystone, Colo , Usa, Mar 11-16, Xvi+564p Academic Press, Inc : New York, N Y , Usa; London, England Illus P325-338,, 1979

Mutations in WSC genes for putative stress receptors result in sensitivity to multiple stress conditions and impairment of Rlm1-dependent gene expression in Saccharomyces cerevisiae. Molecular Genetics and Genomics: Mgg 266(1): 142-155, 2001

Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes. Proceedings of the National Academy of Sciences of the United States of America 81(8): 2431-2434, 1984

The Saccharomyces cerevisiae SPT14 gene is essential for normal expression of the yeast transposon, Ty, as well as for expression of the HIS4 gene and several genes in the mating pathway. Molecular and General Genetics: Mgg 230(1-2): 310-320, 1991

Cloning of HAL1 gene from Saccharomyces cerevisiae and construction of its plant expression vector. Journal of Yangzhou University, Agricultural and Life Sciences Edition 23(4): 27-29, 2002