+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Total sialic acid: an acute phase reactant in cats with a possible role in feline coronavirus infection

Total sialic acid: an acute phase reactant in cats with a possible role in feline coronavirus infection

Canadian Journal of Veterinary Research 73(2): 144-150

The aims of this study were to validate a colorimetric method to measure total sialic acid (TSA) in feline serum and to investigate the serum concentration of TSA in clinically healthy cats seronegative (n = 9) and seropositive (n = 48) for feline coronavirus (FCoV), and in cats affected by feline infectious peritonitis (FIP, n = 28), tumors (n = 20), or inflammation (n = 16). The correlation between TSA and alpha(1)-acid glycoprotein (AGP) was also investigated. The method employed in this study is precise and accurate at TSA levels (in mg/L) commonly encountered in feline serum. No significant differences between seropositive (385.6 +/- 192.2 mg/L) and seronegative (433.5 +/- 179.0 mg/L) cats were detectable, suggesting that the simple infection by FCoVs does not influence TSA levels. Compared with seropositive controls, the concentration of TSA was higher in cats with FIP (556.7 +/- 268.3 mg/L, P = 0.003), tumors (522.5 +/- 294.4 mg/L, P = 0.028), and inflammation (546.8 +/- 208.3 mg/L, P = 0.018). The discriminating power of TSA for FIP is moderate (area under the ROC curve = 0.65) and the likelihood ratio is higher than 3.0 only at high TSA levels. Consequently, TSA could support a diagnosis of FIP only at extremely high serum concentration (> 800 mg/L) or when the pre-test probability of FIP is high. No correlations were found between the TSA and AGP concentrations in cats with FIP, suggesting that sialylated proteins other than AGP are present. Both the antibody titre and the degree of AGP sialylation were negatively correlated with TSA levels, suggesting that increased TSA may contribute to reduce the burden of FCoVs.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 056611125

Download citation: RISBibTeXText

PMID: 19436584

Related references

Changes in some acute phase protein and immunoglobulin concentrations in cats affected by feline infectious peritonitis or exposed to feline coronavirus infection. Veterinary Journal 167(1): 38-44, 2004

Sialic acid--an acute phase reactant and a concomitant of tumors. Zeitschrift für Medizinische Laboratoriumsdiagnostik 32(3-4): 188-192, 1991

Correlative Study of Several Acute Phase Reactant with Particular Referance to Sialic Acid. Pediatrics International 6(2): 34-34, 1964

Canine coronavirus infection in cats; a possible role in feline infectious peritonitis. Advances in Experimental Medicine and Biology 276: 475-479, 1990

Changes of the blood sialic acid and acute phase reactant levels in chronic rheumatoid arthritis. Rinsho Byori. Japanese Journal of Clinical Pathology Suppl 54: 113-119, 1983

Measurement of sialic acid and acute phase reactant proteins in sera of patients with diabetic nephropathy. Journal of Diabetic Complications 2(4): 175-178, 1988

Serum alpha1-acid glycoprotein (AGP) concentration in non-symptomatic cats with feline coronavirus (FCoV) infection. Journal of Feline Medicine and Surgery 9(4): 271-277, 2007

Serum total sialic acid and lipid-associated sialic acid in normal individuals and patients with myocardial infarction, and their relationship to acute phase proteins. Annals of Clinical Biochemistry 30: 383-386, 1993

Antibody-dependent enhancement of feline infectious peritonitis virus infection in feline alveolar macrophages and human monocyte cell line U937 by serum of cats experimentally or naturally infected with feline coronavirus. Journal of Veterinary Medical Science 60(1): 49-55, 1998

Long-term impact on a closed household of pet cats of natural infection with feline coronavirus, feline leukaemia virus and feline immunodeficiency virus. Veterinary Record 146(15): 419-424, 2000

Expression profiles of immune mediators in feline Coronavirus-infected cells and clinical samples of feline Coronavirus-positive cats. Bmc Veterinary Research 13(1): 92, 2017

Discrepancies between feline coronavirus antibody and nucleic acid detection in effusions of cats with suspected feline infectious peritonitis. Research in Veterinary Science 125: 421-424, 2019

Role of sialic acids in feline enteric coronavirus infections. Journal of General Virology 95(Pt 9): 1911-1918, 2014

Feline infectious peritonitis: role of the feline coronavirus 3c gene in intestinal tropism and pathogenicity based upon isolates from resident and adopted shelter cats. Virus Research 165(1): 17-28, 2012

Differential recognition of peptides within feline coronavirus polyprotein 1 ab by sera from healthy cats and cats with feline infectious peritonitis. Virology 532: 88-96, 2019