+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcription factor NF-Y is involved in differentiation of R7 photoreceptor cell in Drosophila



Transcription factor NF-Y is involved in differentiation of R7 photoreceptor cell in Drosophila



Biology Open 1(1): 19-29



The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB and NF-YC. Knockdown of Drosophila NF-YA (dNF-YA) in eye discs with GMR-GAL4 and UAS-dNF-YAIR resulted in a rough eye phenotype and monitoring of differentiation of photoreceptor cells by LacZ expression in seven up-LacZ and deadpan-lacZ enhancer trap lines revealed associated loss of R7 photoreceptor signals. In line with differentiation of R7 being regulated by the sevenless (sev) gene and the MAPK cascade, the rough eye phenotype and loss of R7 signals in dNF-YA-knockdown flies were rescued by expression of the sev gene, or the D-raf gene, a downstream component of the MAPK cascade. The sev gene promoter contains two dNF-Y-binding consensus sequences which play positive roles in promoter activity. In chromatin immunoprecipitation assays with anti-dNF-YA antibody and S2 cells, the sev gene promoter region containing the NF-Y consensus was effectively amplified in immunoprecipitates from transgenic flies by polymerase chain reaction, indicating that dNF-Y is necessary for appropriate sev expression and involved in R7 photoreceptor cell development.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056635500

Download citation: RISBibTeXText

PMID: 23213364

DOI: 10.1242/bio.2011013


Related references

The transcription factor Glass links eye field specification with photoreceptor differentiation in Drosophila. Development 143(8): 1413-1423, 2016

The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth. Development 130(16): 3651-3662, 2003

The Drosophila DOCK family protein sponge is involved in differentiation of R7 photoreceptor cells. Experimental Cell Research 319(14): 2179-2195, 2013

Transcription factors involved in retinogenesis are co-opted by the circadian clock following photoreceptor differentiation. Development 141(13): 2644-2656, 2014

Identification and characterization of mask, a novel protein involved in Corkscrew signaling in Drosophila melanogaster photoreceptor differentiation. Molecular Biology of the Cell 11(Supplement): 62a, 2000

Regulation of Drosophila intestinal stem cell maintenance and differentiation by the transcription factor Escargot. Embo Journal 33(24): 2983-2996, 2015

R7 Photoreceptor Specification in the Developing Drosophila Eye: The Role of the Transcription Factor Deadpan. Plos Genetics 12(7): E1006159, 2017

RZIP, a transcription factor homolog involved in cell differentiation and patterning during the development of Dictyostelium discoideum. Journal of Cellular Biochemistry Supplement 0(19A): 334, 1995

Role of transcription factor Tgif2 in photoreceptor differentiation in the mouse retina. Experimental Eye Research 152: 34-42, 2016

ETS1 encoding a transcription factor involved in B-cell differentiation is recurrently deleted and down-regulated in classical Hodgkin's lymphoma. Haematologica 97(10): 1612-1614, 2013

The neuronal transcription factor erect wing regulates specification and maintenance of Drosophila R8 photoreceptor subtypes. Developmental Biology 381(2): 482-490, 2013

Expression of Drosophila rhodopsins during photoreceptor cell differentiation: insights into R7 and R8 cell subtype commitment. Gene Expression Patterns 6(7): 687-694, 2006

The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio). American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 296(4): R1192-R1201, 2009

Leukemia inhibitory factor blocks expression of Crx and Nrl transcription factors to inhibit photoreceptor differentiation. Investigative Ophthalmology & Visual Science 46(7): 2601-2610, 2005

Sumoylation of bZIP transcription factor NRL modulates target gene expression during photoreceptor differentiation. Journal of Biological Chemistry 285(33): 25637-25644, 2010