+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcriptional assessment by microarray analysis and large-scale meta-analysis of the metabolic capacity of cardiac and skeletal muscle tissues to cope with reduced nutrient availability in Gilthead Sea Bream (Sparus aurata L.)



Transcriptional assessment by microarray analysis and large-scale meta-analysis of the metabolic capacity of cardiac and skeletal muscle tissues to cope with reduced nutrient availability in Gilthead Sea Bream (Sparus aurata L.)



Marine Biotechnology 16(4): 423-435



The effects of nutrient availability on the transcriptome of cardiac and skeletal muscle tissues were assessed in juvenile gilthead sea bream fed with a standard diet at two feeding levels: (1) full ration size and (2) 70 % satiation followed by a finishing phase at the maintenance ration. Microarray analysis evidenced a characteristic transcriptomic profile for each muscle tissue following changes in oxidative capacity (heart > red skeletal muscle > white skeletal muscle). The transcriptome of heart and secondly that of red skeletal muscle were highly responsive to nutritional changes, whereas that of glycolytic white skeletal muscle showed less ability to respond. The highly expressed and nutritionally regulated genes of heart were mainly related to signal transduction and transcriptional regulation. In contrast, those of white muscle were enriched in gene ontology (GO) terms related to proteolysis and protein ubiquitination. Microarray meta-analysis using the bioinformatic tool Fish and Chips ( http://fishandchips.genouest.org/index.php ) showed the close association of a representative cluster of white skeletal muscle with some of cardiac and red skeletal muscle, and many GO terms related to mitochondrial function appeared to be common links between them. A second round of cluster comparisons revealed that mitochondria-related GOs also linked differentially expressed genes of heart with those of liver from cortisol-treated gilthead sea bream. These results show that mitochondria are among the first responders to environmental and nutritional stress stimuli in gilthead sea bream, and functional phenotyping of this cellular organelle is highly promising to obtain reliable markers of growth performance and well-being in this fish species.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056636514

Download citation: RISBibTeXText

PMID: 24626932

DOI: 10.1007/s10126-014-9562-3


Related references

Molecular characterization of gilthead sea bream (Sparus aurata) lipoprotein lipase. Transcriptional regulation by season and nutritional condition in skeletal muscle and fat storage tissues. Comparative Biochemistry and Physiology. Part B Biochemistry and Molecular Biology 142(2): 224-232, 2005

Characterisation and expression analysis of cathepsins and ubiquitin-proteasome genes in gilthead sea bream (Sparus aurata) skeletal muscle. Bmc Research Notes 8: 149, 2015

Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata). Comparative Biochemistry and Physiology. Part B Biochemistry and Molecular Biology 199: 50-57, 2016

Metabolic and transcriptional responses of gilthead sea bream (Sparus aurata L.) to environmental stress: new insights in fish mitochondrial phenotyping. General and Comparative Endocrinology 205: 305-315, 2014

Co-expression Analysis of Sirtuins and Related Metabolic Biomarkers in Juveniles of Gilthead Sea Bream ( Sparus aurata ) With Differences in Growth Performance. Frontiers in Physiology 9: 608, 2018

Stable isotope analysis combined with metabolic indices discriminates between gilthead sea bream (Sparus aurata) fingerlings produced in various hatcheries. Journal of Agricultural and Food Chemistry 59(18): 10261-10270, 2011

Metabolic Effects of Insulin and IGFs on Gilthead Sea Bream (Sparus aurata) Muscle Cells. Frontiers in Endocrinology 3: 55, 2012

Fast skeletal muscle transcriptome of the gilthead sea bream (Sparus aurata) determined by next generation sequencing. Bmc Genomics 13: 181, 2012

Metabolic rate and tissue reserves in gilthead sea bream (Sparus aurata) under thermal fluctuations and fasting and their capacity for recovery. Canadian Journal of Fisheries and Aquatic Sciences 64(7): 1034-1042, 2007

Distinct role of insulin and IGF-I and its receptors in white skeletal muscle during the compensatory growth of gilthead sea bream (Sparus aurata). Aquaculture 267(1/4): 188-198, 2007

Metabolic and molecular stress responses of gilthead seam bream Sparus aurata during exposure to low ambient temperature: an analysis of mechanisms underlying the winter syndrome. Journal of Comparative Physiology. B Biochemical Systemic and Environmental Physiology 180(7): 1005-1018, 2010

Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata). Comparative Biochemistry and Physiology. Part D Genomics and Proteomics 2(3): 187-199, 2007

Effects of dietary soybean oil concentration on growth, nutrient utilization and muscle fatty acid composition of gilthead sea bream (Sparus aurata L.). Aquaculture Research 38(1): 76-81, 2007

Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles. Comparative Biochemistry and Physiology. Part A Molecular and Integrative Physiology 191: 9, 2016

Dietary arginine surplus does not improve intestinal nutrient absorption capacity, amino acid metabolism and oxidative status of gilthead sea bream (Sparus aurata) juveniles. Aquaculture 464: 480-488, 2016