+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions

Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions

Journal of Leukocyte Biology 84(1): 143-151

In human skin, two main populations of dendritic cells (DC) can be discriminated: dermal DC (DDC) and epidermal Langerhans cells (LC). Although extensively studied, most of the knowledge about DDC and LC phenotype and function is obtained from studying DDC and LC cultured in vitro or DDC and LC migrated from skin explants. These studies have left the exact relationship between steady-state human LC and DDC unclear: in particular, whether CD1a+ DDC represent migrated LC or whether they constitute a separate subset. To gain further insight in the kinship between skin-resident CD1a+ DDC and LC, we analyzed CD1a+ DDC and LC, isolated from steady-state skin samples, by high-density microarray analysis. Results show that the CD1a+ DDC specifically express markers associated with DDC phenotype, such as the macrophage mannose receptor, DC-specific ICAM-grabbing nonintegrin, the scavenger receptor CD36, coagulation factor XIIIa, and chemokine receptor CCR5, whereas LC specifically express Langerin, membrane ATPase (CD39), and CCR6, all hallmarks of the LC lineage. In addition, under steady-state conditions, both DC subsets display a strikingly different activation status, indicative of distinct functional properties. CD1a+ DDC exhibit a more activated, proinflammatory, migratory, and T cell-stimulatory profile, as compared with LC, whereas LC mainly express molecules involved in cell adhesion and DC retention in the epidermis. In conclusion, transcriptional profiling is consistent with the notion that CD1a+ DDC and LC represent two distinct DC subsets but also that under steady-state conditions, CD1a+ DDC and epidermal LC represent opposites of the DC activation spectrum.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 056637192

Download citation: RISBibTeXText

PMID: 18436579

DOI: 10.1189/jlb.1107750

Related references

Epitopes for CD1a, CD1b, and CD1c antigens are differentially mapped on Langerhans cells, dermal dendritic cells, keratinocytes, and basement membrane zone in human skin. Journal of the American Academy of Dermatology 27(3): 419-426, 1992

Langerhans cells (CD1a and CD207), dermal dendrocytes (FXIIIa) and plasmacytoid dendritic cells (CD123) in skin lesions of leprosy patients. Microbial Pathogenesis 91: 18-25, 2016

Murine epidermal Langerhans cells and langerin-expressing dermal dendritic cells are unrelated and exhibit distinct functions. Proceedings of the National Academy of Sciences of the United States of America 106(9): 3312-3317, 2009

Langerhans cells, dermal dendritic cells and monocyte-derived dendritic cells are distinct subsets of myeloid dendritic cells defined by diverse functional properties. Archives of Dermatological Research 295(8-9): 342, 2004

Dynamics and function of Langerhans cells in vivo: dermal dendritic cells colonize lymph node areas distinct from slower migrating Langerhans cells. Immunity 22(5): 643-654, 2005

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice. Biological and Pharmaceutical Bulletin 39(8): 1309-1318, 2016

Immunohistochemical study of epidermal Langerhans cells and dermal dendritic cells in benign and malignant skin lesions characterized by a dermal lymphoid infiltrate consisting either of B-cells or T-cells. Virchows Archiv. A Pathological Anatomy and Histopathology 411(4): 337-343, 1987

CD1a+ and CD1a- monocyte-derived dendritic cells In vitro model for dermal dendritic cells?. Journal of Investigative Dermatology 109(2): 257, 1997

Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin. Journal of Immunology 168(9): 4361-4371, 2002

Relay of herpes simplex virus between Langerhans cells and dermal dendritic cells in human skin. Plos Pathogens 11(4): E1004812, 2015

Epidermal Langerhans' cells and dermal dendritic cells in human fetal and neonatal skin: an immunohistochemical study. Pediatric Dermatology 4(1): 11-17, 1987

Puva therapy decreases hla dr positive cd1a positive langerhans cells and epidermal cell antigen presenting capacity in human skin but flow cytometrically sorted residual hla dr positive cd1a positive langerhans cells exhibit normal alloantigen presenting function. British Journal of Dermatology 120(3): 329-340, 1989

Effects of solar ultraviolet radiation on engineered human skin equivalent containing both Langerhans cells and dermal dendritic cells. Tissue Engineering 13(11): 2667-2679, 2007

Brugia malayi infective larvae fail to activate Langerhans cells and dermal dendritic cells in human skin. Parasite Immunology 37(2): 79-91, 2015

Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells. Journal of Leukocyte Biology 97(4): 627-634, 2015