+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Transcriptional regulation of the murine Presenilin-2 gene reveals similarities and differences to its human orthologue

Transcriptional regulation of the murine Presenilin-2 gene reveals similarities and differences to its human orthologue

Gene 446(2): 81-89

ISSN/ISBN: 0378-1119

PMID: 19573580

DOI: 10.1016/j.gene.2009.06.015

Inherited Presenilin-2 mutations cause familial Alzheimer's disease, and its regulation may play a role in sporadic cases. The human Presenilin-2 (PSEN2) regulatory region includes two separate promoters modulated by Egr-1, a transcription factor involved in learning and memory. To enable in-vivo analysis of Presenilin-2 regulation, we characterized the murine Presenilin-2 (Psen2) promoter. We identified novel Psen2 Transcription start sites (TSSs) 10 kb upstream of previously reported sites, along with two new alternatively transcribed exons (1A, and 1BC) in the 5' untranslated region. Transcripts initiating in Exon 1A are ubiquitous, whereas exon 1BC-initiated transcripts are non-neuronal. Only the sequence surrounding exon 1A, which includes homologous sequences to the human PSEN2 promoter, harbored significant promoter activity. Sequences upstream of exon 1A and a downstream enhancer were specifically important in neuronal cells, but similar to the human promoter, the murine promoter was characteristic of a housekeeping gene, and its activity depended on Sp1 binding. Egr-1 did not bind the murine promoter. Egr-1 over-expression and down-regulation, as well as in-vivo examination of Egr-1 and Psen2 expression during fear conditioning in mice, showed that Egr-1 does not regulate the murine Psen2 promoter. Differential Psen2 regulation in human and mouse has implications for Alzheimer disease mouse models.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 056637763

Download citation: RISBibTeXText

Related references

Similarities and differences in the transcriptional regulation of the leptin gene promoter in gastric and adipose cells. Febs Letters 579(9): 1911-1916, 2005

Global transcriptional profiling reveals similarities and differences between human stem cell-derived cardiomyocyte clusters and heart tissue. Physiological Genomics 44(4): 245-258, 2012

Kluyveromyces lactis HIS4 transcriptional regulation: similarities and differences to Saccharomyces cerevisiae HIS4 gene. Febs Letters 458(1): 72-76, 1999

The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its human counterpart. Gene 158(2): 253-256, 1995

Transcriptional regulation of the presenilin-2 gene. Society for Neuroscience Abstracts 23(1-2): 2168, 1997

Transcriptional regulation of the presenilin-1 gene. Society for Neuroscience Abstracts 23(1-2): 823, 1997

Alternative initiation of transcription of the human presenilin 1 gene in SH-SY5Y and SK-N-SH cells. The role of Ets factors in the regulation of presenilin 1. European Journal of Biochemistry 271(22): 4485-4494, 2004

Transcriptional regulation of the mouse presenilin-1 gene. Journal of Biological Chemistry 272(38): 23489-23497, 1997

Transcriptional regulation of the presenilin-1 gene implication in Alzheimers disease. Frontiers in Bioscience: 13, 2008

Transcriptional regulation of the presenilin-1 gene: implication in Alzheimer's disease. Frontiers in Bioscience: a Journal and Virtual Library 13: 822-832, 2008

Transcriptional regulation and the negative feedback mechanism of the presenilin-1 gene. Society for Neuroscience Abstracts 24(1-2): 473, 1998

Differences in human VH gene transcriptional regulation. FASEB Journal 16(4): A694, 2002

Transcriptional regulation of the presenilin-1 gene controls gamma-secretase activity. Frontiers in Bioscience 2(1): 22-35, 2010

Expression and regulation of murine SPINK12, a potential orthologue of human LEKTI2. Experimental Dermatology 20(11): 905-910, 2011

Telomerase inhibition in RenCa, a murine tumor cell line with short telomeres, by overexpression of a dominant negative mTERT mutant, reveals fundamental differences in telomerase regulation between human and murine cells. Cancer Research 61(14): 5580-5586, 2001