Two-Step Purification of Cordycepin from Cordyceps Millitaris by High-Speed Countercurrent Chromatography

Ju, X.; Sun, Y.; Cao, X.; Jiang, J.; Zhang, T.; Ito, Y.

Journal of Liquid Chromatography and Related Technologies 32(16): 2417-2423


ISSN/ISBN: 1082-6076
PMID: 20046921
DOI: 10.1080/10826070903188153
Accession: 056721631

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Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis.