Tyr-51 is the proton donor-acceptor for NAD (H) -dependent interconversion of xylose and xylitol by Candida tenuis xylose reductase (AKR2B5)
Pival, S.L.; Klimacek, M.; Kratzer, R.; Nidetzky, B.
Febs Letters 582(29): 4095-4099
ISSN/ISBN: 0014-5793 PMID: 19026644 DOI: 10.1016/j.febslet.2008.11.003
Substitution of active-site Tyr-51 by Ala (Y51A) disrupted the activity of Candida tenuis xylose reductase by six orders of magnitude. External bromide brought about unidirectional rate enhancement ( approximately 2x10(3)-fold at 300mM) for NAD(+)-dependent xylitol oxidation by Y51A. Activity of the wild-type reductase was dependent on a single ionizable protein group exhibiting a pK of 9.2+/-0.1 and 7.3+/-0.3 in the holo-enzyme bound with NADH and NAD(+), respectively. This group which had to be protonated for xylose reduction and unprotonated for xylitol oxidation was eliminated in Y51A, consistent with a catalytic acid-base function of Tyr-51. Bromide may complement the xylitol dehydrogenase activity of Y51A by partly restoring the original hydrogen bond between the reactive alcohol and the phenolate of Tyr-51.