+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ultra high resolution SFC-MS as a high throughput platform for metabolic phenotyping: application to metabolic profiling of rat and dog bile



Ultra high resolution SFC-MS as a high throughput platform for metabolic phenotyping: application to metabolic profiling of rat and dog bile



Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 966: 200-207



Ultra high resolution SFC-MS (on sub-2μm particles) coupled to mass spectrometry has been evaluated for the metabolic profiling of rat and dog bile. The selectivity of the SFC separation differed from that seen in previous reversed-phase UPLC-MS studies on bile, with the order of elution for analytes such as e.g., the bile acids showing many differences. The chromatography system showed excellent stability, reproducibility and robustness with relative standard deviation of less than 1% for retention time obtained over the course of the analysis. SFC showed excellent chromatographic performance with chromatographic peak widths in the order of 3s at the base of the peak. The use of supercritical fluid carbon dioxide as a mobile phase solvent also reduced the overall consumption of organic solvent by a factor of 3 and also reduced the overall analysis time by a factor of 30% compared to reversed-phase gradient LC. SFC-MS appear complementary to RPLC for the metabolic profiling of complex samples such as bile.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056734756

Download citation: RISBibTeXText

PMID: 24815366

DOI: 10.1016/j.jchromb.2014.04.017


Related references

A novel platform for automated high-throughput fluxome profiling of metabolic variants. Metabolic Engineering 25: 8, 2014

Development of a Rapid Microbore Metabolic Profiling Ultraperformance Liquid Chromatography-Mass Spectrometry Approach for High-Throughput Phenotyping Studies. Analytical Chemistry 88(11): 5742-5751, 2016

Ultra-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Direct Infusion-High-Resolution Mass Spectrometry for Combined Exploratory and Targeted Metabolic Profiling of Human Urine. Journal of Proteome Research 17(10): 3492-3502, 2018

Microalgal Metabolic Network Model Refinement through High-Throughput Functional Metabolic Profiling. Frontiers in Bioengineering and Biotechnology 2: 68, 2014

A high-throughput bioanalytical platform using automated infusion for tandem mass spectrometric method optimization and its application in a metabolic stability screen. Rapid Communications in Mass Spectrometry 23(11): 1579-1591, 2009

High resolution "ultra performance" liquid chromatography coupled to oa-TOF mass spectrometry as a tool for differential metabolic pathway profiling in functional genomic studies. Journal of Proteome Research 4(2): 591-598, 2005

Metabolic profiling of icariin in rat feces, urine, bile and plasma after oral administration using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 168: 155-162, 2019

Ultra-performance liquid chromatography coupled to time-of-flight mass spectrometry for robust, high-throughput quantitative analysis of an automated metabolic stability assay, with simultaneous determination of metabolic data. Rapid Communications in Mass Spectrometry 20(5): 851-857, 2006

High throughput, high precision metabolic profiling approaches to optimise the nutritional value of crops and crop-based foods: The EU DEVELONUTRI project. Journal of Biotechnology 150(Supp-S): 65-66, 2010

High-throughput screening of cellular features using high-resolution light-microscopy; application for profiling drug effects on cell adhesion. Journal of Structural Biology 158(2): 233-243, 2007

High-Throughput Microbore UPLC-MS Metabolic Phenotyping of Urine for Large-Scale Epidemiology Studies. Journal of Proteome Research 14(6): 2714-2721, 2015

Metabolic profiling of Gegenqinlian decoction in rat plasma, urine, bile and feces after oral administration by ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry. Journal of Chromatography B 1079: 69-84, 2018

Metabolic profiling of Gegenqinlian decoction in rat plasma, urine, bile and feces after oral administration by ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 1079: 69-84, 2018

Metabolic profiling of nuciferine in rat urine, plasma, bile and feces after oral administration using ultra-high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 140: 71-80, 2017

Driving efficiency in a high-throughput metabolic stability assay through a generic high-resolution accurate mass method and automated data mining. Protein and Cell 2(8): 680-688, 2011