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Use of cell culture to screen sunflower germplasm for resistance to Phomopsis brown/gray stem spot



Use of cell culture to screen sunflower germplasm for resistance to Phomopsis brown/gray stem spot



Plant Cell Reports 7(7): 528-530



Resistance to Phomopsis sp. (Diaporthe sp.) brown/gray stem spot was confirmed by screening sunflower calli on shoot induction media amended with fungal filtrate. Calli of sunflower genotypes OCMS 74 and NS-H-45, which show resistance to the disease in field trials, remained viable on media with 15% (v/v) fungal filtrate, while calli of field susceptible genotypes RHA 273 and PAG/SF 103 were killed on media with as little as 7.5% fungal filtrate. Fresh weight of calli of all genotypes was significantly reduced by 2.5% fungal filtrate, and calli of all genotypes were killed by filtrate concentrations of 20%. These in vitro results corroborate prior field observations for disease reaction of these genotypes.

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Accession: 056795401

Download citation: RISBibTeXText

PMID: 24240408

DOI: 10.1007/bf00272748


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