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Use of guanidinopropyl-modified si RNAs to silence gene expression

Use of guanidinopropyl-modified si RNAs to silence gene expression

Methods in Molecular Biology 1218: 217-249

ISSN/ISBN: 1064-3745

PMID: 25319654

DOI: 10.1007/978-1-4939-1538-5_13

Silencing gene expression by harnessing the RNA interference (RNAi) pathway with short interfering RNAs (siRNAs) has useful analytical and potentially therapeutic application. To augment silencing efficacy of siRNAs, chemical modification has been employed to improve stability, target specificity, and delivery to target tissues. siRNAs incorporating guanidinopropyl (GP) moieties have demonstrated enhanced target gene silencing in cell culture and in vivo models of hepatitis B virus replication. Here we describe the synthesis of GP-modified siRNAs and use of 5' rapid amplification of cDNA ends (5' RACE) to verify an RNAi-mediated mechanism of action of these novel chemically modified siRNAs.

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Accession: 056798199

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