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Using Galleria mellonella-Candida albicans infection model to evaluate antifungal agents

Using Galleria mellonella-Candida albicans infection model to evaluate antifungal agents

Biological and Pharmaceutical Bulletin 36(9): 1482-1487

Candida albicans is the most common fungal pathogen. Galleria mellonella is widely used as an infection model host. Nevertheless, the G. mellonella-C. albicans infection model had not been optimized for drug evaluation before this study. In this work, we revealed that 5 × 10(5) colony forming unit (CFU)/larva was a suitable inoculum to optimize the G. mellonella-C. albicans infection model in order to evaluate antifungal agents. Using our optimized model, the antifungal effect of fluconazole, amphotericin B and flucytosine, and the synergy between amphotericin B and flucytosine were successfully verified. Thus, this study provides a rapid, inexpensive and reliable way to evaluate antifungals in vivo.

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Accession: 056810744

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PMID: 23995660

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