+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Utilization of Jatropha deoiled seed cake for production of cellulases under solid-state fermentation

Utilization of Jatropha deoiled seed cake for production of cellulases under solid-state fermentation

Bioprocess and Biosystems Engineering 35(8): 1343-1353

Toxic waste generated by Jatropha seed cake after utilization of biodiesel on one hand has stimulated the need to develop new technologies to treat the waste and on the other, forced us to reevaluate the efficient utilization of its nutritive potential for production of various high-value compounds and its conversion to non-toxic forms which could be used as animal feed stock. In this study, Jatropha seed cake was used for production of cellulases by new isolate of Thermoascus aurantiacus under solid-state fermentation. The interaction of nitrogen source concentration, moisture ratio, initial pH of the medium and inoculum size was investigated and modelled using response surface methodology (RSM) using Box-Behnken Design (BBD). Under optimized conditions endo-β-1,4-glucanase, β-glucosidase and filter paper activities were found to be 124.44, 28.86, 4.87 U/g of substrate, respectively. Characterization of endo-β-1,4-glucanase, β-glucosidase was done after partial purification by ammonium sulfate fractionation followed by desalting. The endo-β-1,4-glucanase and β-glucosidase showed maximum activity at 70 °C and pH 4. Saccharification studies performed with different lignocellulosic substrates showed that sugar cane bagasse was most susceptible to enzymatic hydrolysis. The study suggests that Jatropha seed cake can be used as a viable nutrient source for cellulase production without any pretreatment under solid-state fermentation by T. aurantiacus.

(PDF emailed within 0-6 h: $19.90)

Accession: 056824176

Download citation: RISBibTeXText

PMID: 22451079

DOI: 10.1007/s00449-012-0723-3

Related references

Production of Sporotrichum thermophile xylanase by solid state fermentation utilizing deoiled Jatropha curcas seed cake and its application in xylooligosachharide synthesis. Bioresource Technology 153: 126-130, 2014

Degradation of phorbol esters by Pseudomonas aeruginosa PseA during solid-state fermentation of deoiled Jatropha curcas seed cake. Bioresource Technology 102(7): 4815-4819, 2011

Solid-state fermentation of Jatropha seed cake for optimization of lipase, protease and detoxification of anti-nutrients in Jatropha seed cake using Aspergillus versicolor CJS-98. Journal of Bioscience and Bioengineering 117(2): 208-214, 2014

Utilization of deoiled Jatropha curcas seed cake for production of xylanase from thermophilic Scytalidium thermophilum. Bioresource Technology 102(2): 1722-1726, 2011

Study on Utilization of Detoxified Jatropha curcas Seed Cake Subjected to Solid State Fermentation as a Dietary Supplement in Wistar Rats. Recent Patents on Food, Nutrition & Agriculture 8(3): 190-198, 2016

Jatropha curcas seed cake as substrate for production of xylanase and cellulase by Aspergillus niger FGSCA733 in solid-state fermentation. Industrial Crops and Products 37(1): 118-123, 2012

Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Bioresource Technology 99(6): 1729-1735, 2007

Bio-detoxification of phorbol esters and other anti-nutrients of Jatropha curcas seed cake by fungal cultures using solid-state fermentation. Applied Biochemistry and Biotechnology 172(5): 2747-2757, 2014

Jatropha curcasseed cake as substrate for production of xylanase and cellulase byAspergillus nigerFGSCA733 in solid-state fermentation. 2012

Detoxification of Jatropha curcas seed cake in solid-state fermentation of newly isolated endophytic strain and nutrition assessment for its potential utilizations. International Biodeterioration & Biodegradation 109: 202-210, 2016

Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation. Journal of Environmental Management 91(10): 2019-2027, 2010

Effect of pressure on the hydropyrolysis of Jatropha seed deoiled cake. Journal of Material Cycles and Waste Management 15(3): 328-334, 2013

2-Pyrrolidone synthesis from γ-aminobutyric acid produced by Lactobacillus brevis under solid-state fermentation utilizing toxic deoiled cottonseed cake. Bioprocess and Biosystems Engineering (): -, 2016

Purification and characterization of Pseudomonas aeruginosa lipase produced by SSF of deoiled Jatropha seed cake. Biocatalysis and Agricultural Biotechnology 2(1): 32-37, 2013

Statistical optimization of cellulases production by Aspergillus niger HQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan. Annals of Microbiology 62(2): 629-645, 2012