+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Velocity estimates for signal propagation leading to systemic jasmonic acid accumulation in wounded Arabidopsis



Velocity estimates for signal propagation leading to systemic jasmonic acid accumulation in wounded Arabidopsis



Journal of Biological Chemistry 284(50): 34506-34513



The wound response prohormone jasmonic acid (JA) accumulates rapidly in tissues both proximal and distal to injury sites in plants. Using quantitative liquid chromatography-mass spectrometry after flash freezing of tissues, we found that JA accumulated within 30 s of injury in wounded Arabidopsis leaves (p = 3.5 e(-7)). JA augmentation distal to wounds was strongest in unwounded leaves with direct vascular connections to wounded leaves wherein JA levels increased significantly within 120 s of wounding (p = 0.00027). This gave conservative and statistically robust temporal boundaries for the average velocity of the long distance signal leading to distal JA accumulation in unwounded leaves of 3.4-4.5 cm min(-1). Like JA, transcripts of the JA synthesis gene LIPOXYGENASE2 (LOX2) and the jasmonate response gene JAZ10.3 also accumulated to higher levels in directly interconnected leaves than in indirectly connected leaves. JA accumulation in a lox2-1 mutant plant was initiated rapidly after wounding then slowed progressively compared with the wild type (WT). Despite this, JAZ10.3 expression in the two genotypes was similar. Free cyclopentenone jasmonate levels were similar in both resting WT and lox2-1. In contrast, bound cyclopentenone jasmonates (arabidopsides) were far lower in lox2-1 than in the WT. The major roles of LOX2 are to generate arabidopsides and the large levels of JA that accumulate proximal to the wound. LOX2 is not essential for some of the most rapid events elicited by wounding.

(PDF emailed within 0-6 h: $19.90)

Accession: 056858317

Download citation: RISBibTeXText

PMID: 19846562

DOI: 10.1074/jbc.M109.061432


Related references

Kinetics of the accumulation of jasmonic acid and its derivatives in systemic leaves of tobacco (Nicotiana tabacum cv. Xanthi nc) and translocation of deuterium-labeled jasmonic acid from the wounding site to the systemic site. Bioscience, Biotechnology, and Biochemistry 73(9): 1962-1970, 2010

Plastid omega3-fatty acid desaturase-dependent accumulation of a systemic acquired resistance inducing activity in petiole exudates of Arabidopsis thaliana is independent of jasmonic acid. Plant Journal 54(1): 106-117, 2007

The glycosyltransferase UGT76E1 significantly contributes to 12- O -glucosylpyranosyl-jasmonic acid formation in wounded Arabidopsis thaliana leaves. Journal of Biological Chemistry 2019, 2019

Gene expression profiling in wounded and systemic leaves of Fagus sylvatica reveals up-regulation of ethylene and jasmonic acid signalling. Plant Biology 13(3): 445-452, 2011

A defect in glyoxysomal fatty acid beta-oxidation reduces jasmonic acid accumulation in Arabidopsis. Plant Physiology and Biochemistry 43(6): 603-609, 2005

Systemic effects on oxidative enzymes in Phaseolus vulgaris leaves that have been wounded by the grasshopper Melanoplus differentialis (Thomas) or have had a foliar application of jasmonic acid. International Journal of Plant Sciences 163(2): 317-328, 2002

Involvement of phospholipase D in wound-induced accumulation of jasmonic acid in arabidopsis. Plant Cell 12(11): 2237-2246, 2000

The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis. Plant Cell 16(8): 2117-2127, 2004

Fatty acid-amino acid conjugates are essential for systemic activation of salicylic acid-induced protein kinase and accumulation of jasmonic acid in Nicotiana attenuata. Bmc Plant Biology 14: 326, 2015

A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 92(19): 8675-8679, 1995

Jasmonic acid and glucose synergistically modulate the accumulation of glucosinolates in Arabidopsis thaliana. Journal of Experimental Botany 64(18): 5707-5719, 2014

Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence. Plant Cell 26(1): 230-245, 2014

Xenobiotic- and jasmonic acid-inducible signal transduction pathways have become interdependent at the Arabidopsis CYP81D11 promoter. Plant Physiology 159(1): 391-402, 2012

Jasmonic acid enhancement of anthocyanin accumulation is dependent on phytochrome A signaling pathway under far-red light in Arabidopsis. Biochemical and Biophysical Research Communications 454(1): 78-83, 2015

Colonization of Arabidopsis roots by Trichoderma atroviride promotes growth and enhances systemic disease resistance through jasmonic acid/ethylene and salicylic acid pathways. European Journal of Plant Pathology 131(1): 15-26, 2011