Zinc-dependent binding between peptides derived from rainbow trout CD8alpha and LCK
Hayashi, N.; Takeuchi, M.; Nakanishi, T.; Hashimoto, K.; Dijkstra, J.M.
Fish and Shellfish Immunology 28(1): 72-76
ISSN/ISBN: 1095-9947 PMID: 19815107 DOI: 10.1016/j.fsi.2009.09.020
The cytoplasmic tail of mammalian CD8alpha binds the kinase LCK in a zinc-dependent manner. In analogy with a previous study for humans (Kim et al., 2003) peptides were synthesized from rainbow trout CD8alpha and LCK. Surface plasmon resonance (SPR) analysis indicated that also in fish these molecules bind to each other in a zinc-dependent manner.