+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

α-1,6-Mannosylation of N-linked oligosaccharide present on cell wall proteins is required for their incorporation into the cell wall in the filamentous fungus Neurospora crassa

α-1,6-Mannosylation of N-linked oligosaccharide present on cell wall proteins is required for their incorporation into the cell wall in the filamentous fungus Neurospora crassa

Eukaryotic Cell 9(11): 1766-1775

ISSN/ISBN: 1535-9778

PMID: 20870880

DOI: 10.1128/ec.00134-10

The enzyme α-1,6-mannosyltransferase (OCH-1) is required for the synthesis of galactomannans attached to the N-linked oligosaccharides of Neurospora crassa cell wall proteins. The Neurospora crassa och-1 mutant has a tight colonial phenotype and a defective cell wall. A carbohydrate analysis of the och-1 mutant cell wall revealed a 10-fold reduction in the levels of mannose and galactose and a total lack of 1,6-linked mannose residues. Analysis of the integral cell wall protein from wild-type and och-1 mutant cells showed that the mutant cell wall had reduced protein content. The och-1 mutant was found to secrete 18-fold more protein than wild-type cells. Proteomic analysis of the proteins released by the mutant into the growth medium identified seven of the major cell wall proteins. Western blot analysis of ACW-1 and GEL-1 (two glycosylphosphatidylinositol [GPI]-anchored proteins that are covalently integrated into the wild-type cell wall) showed that high levels of these proteins were being released into the medium by the och-1 mutant. High levels of ACW-1 and GEL-1 were also released from the och-1 mutant cell wall by subjecting the wall to boiling in a 1% SDS solution, indicating that these proteins are not being covalently integrated into the mutant cell wall. From these results, we conclude that N-linked mannosylation of cell wall proteins by OCH-1 is required for their efficient covalent incorporation into the cell wall.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 056996722

Download citation: RISBibTeXText

Related references

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa. Proceedings of the National Academy of Sciences of the United States of America 106(52): 22157-22162, 2009

The Genetics and Biochemistry of Cell Wall Structure and Synthesis in Neurospora crassa , a Model Filamentous Fungus. Frontiers in Microbiology 10: 2294, 2019

Studies on cell wall polysaccharides from Neurospora crassa. Part II. Change of the structure of cell wall .BETA.-1,3-D-glucan with the growth of Neurospora crassa cells. Agricultural and Biological Chemistry 48(4): 1041-1047, 1984

Neurospora crassa family GH72 glucanosyltransferases function to crosslink cell wall glycoprotein N-linked galactomannan to cell wall lichenin. Fungal Genetics and Biology: Fg and B 123: 60-69, 2019

A proteomic and genetic analysis of the Neurospora crassa conidia cell wall proteins identifies two glycosyl hydrolases involved in cell wall remodeling. Fungal Genetics and Biology: Fg and B 94: 47-53, 2016

Studies on the cell wall of Neurospora crassa. Part V Purification and some properties of two wall-associated (.BETA.1,3)glucanases from Neurospora crassa cells. Agricultural and Biological Chemistry 50(10): 2461-2467, 1986

Mutational analysis of the glycosylphosphatidylinositol (GPI) anchor pathway demonstrates that GPI-anchored proteins are required for cell wall biogenesis and normal hyphal growth in Neurospora crassa. Eukaryotic Cell 5(3): 587-600, 2006

slime, the wall-less variant of Neurospora crassa: The loss of cell-wall assembly is not due to the loss of cell-wall primer or template. Experimental Mycology 6(3): 203-209, 1982

Cell wall assembly of Neurospora crassa: lack of evidence for preexisting cell wall acting as primer or template. Developmental Biology 97(1): 245-249, 1983

Cell wall deficiency in "slime" strains of Neurospora crassa: osmotic inhibition of cell wall synthesis and beta-D-glucan synthase activity. Brazilian Journal of Medical and Biological Research 27(12): 2843-2857, 1994

Cell wall assembly of neurospora crassa isolation and analysis of cell wall less mutants. Current Microbiology 21(4): 233-242, 1990

Neurospora crassa 1,3-α-glucan synthase, AGS-1, is required for cell wall biosynthesis during macroconidia development. Microbiology 160(Part 8): 1618-1627, 2014

Mannosyltransferase is required for cell wall biosynthesis, morphology and control of asexual development in Neurospora crassa. Mycologia 97(4): 872-879, 2005

WSC-1 and HAM-7 are MAK-1 MAP kinase pathway sensors required for cell wall integrity and hyphal fusion in Neurospora crassa. Plos one 7(8): E42374, 2012

The Neurospora crassa dfg5 and dcw1 genes encode α-1,6-mannanases that function in the incorporation of glycoproteins into the cell wall. Plos one 7(6): E38872, 2012