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A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface

Cell 163(6): 1484-1499

ISSN/ISBN: 0092-8674

PMID: 26638075

DOI: 10.1016/j.cell.2015.10.065

The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a number of protein modules in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive interplay between these processes. By monitoring dynamic changes in the centrosome-cilium protein interaction landscape during ciliogenesis, we also identify satellite proteins that support cilia formation. Systematic profiling of proximity interactions combined with functional analysis thus provides a rich resource for better understanding human centrosome and cilia biology. Similar strategies may be applied to other complex biological structures or pathways.

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Accession: 057027615

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