EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface


A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface



Cell 163(6): 1484-1499



ISSN/ISBN: 0092-8674

PMID: 26638075

DOI: 10.1016/j.cell.2015.10.065

The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a number of protein modules in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive interplay between these processes. By monitoring dynamic changes in the centrosome-cilium protein interaction landscape during ciliogenesis, we also identify satellite proteins that support cilia formation. Systematic profiling of proximity interactions combined with functional analysis thus provides a rich resource for better understanding human centrosome and cilia biology. Similar strategies may be applied to other complex biological structures or pathways.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057027615

Download citation: RISBibTeXText

Related references

Co-evolutionary landscape at the interface and non-interface regions of protein-protein interaction complexes. Computational and Structural Biotechnology Journal 19: 3779-3795, 2021

Transcriptional program of ciliated epithelial cells reveals new cilium and centrosome components and links to human disease. Plos one 7(12): E52166, 2012

Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function. Molecular Biology of the Cell 25(19): 2919-2933, 2014

Cell Cycle Regulation of the Centrosome and Cilium. Drug Discovery Today. Disease Mechanisms 10(3-4): E119-E124, 2013

The Centrosome and the Primary Cilium: the Yin and Yang of a Hybrid Organelle. Cells 8(7), 2019

The dynamic cilium in human diseases. Pathogenetics 2(1): 3, 2009

Soil-structure interface effects on dynamic interaction analysis of reinforced concrete lifelines - Les effets d'interface sol-structure sur l'analyse de l'intéraction dynamique des infrastructures en béton renforcé. International Conference on Soil Dynamics and Earthquake Engineering 3: 89-101, 1987

Centrosome fine ultrastructure of the osteocyte mechanosensitive primary cilium. Microscopy and Microanalysis: the Official Journal of Microscopy Society of America Microbeam Analysis Society Microscopical Society of Canada 18(6): 1430-1441, 2012

Soil-structure interface effects on dynamic interaction analysis of reinforced concrete lifelines - Effets d'interface sol-structure sur les calculs d'interaction dynamique pour réseaux enterrés en béton armé. Soil Dynamics and Earthquake Engineering (1984) 8(1): 32-42, 1989

Cilium, centrosome and cell cycle regulation in polycystic kidney disease. Biochimica et Biophysica Acta 1812(10): 1263-1271, 2011

The cellular and developmental program connecting the centrosome and cilium duplication cycle. Seminars in Cell and Developmental Biology 21(2): 139-141, 2010

Cell polarity and the innovation of the primary cilium/centrosome organ in Metazoa. Medecine Sciences: M/S 35(5): 452-461, 2019

A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex. Cells 9(6), 2020

Cilium evolution: identification of a novel protein, nematocilin, in the mechanosensory cilium of Hydra nematocytes. Molecular Biology and Evolution 25(9): 2009-2017, 2008

Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration. Science Advances 6(36), 2020