+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman-ARMS Followed a Strict Control System

Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman-ARMS Followed a Strict Control System

Journal of Clinical Laboratory Analysis 30(4): 315-318

Hepatitis B virus (HBV) G1896A mutation was associated with HBeAg seronegativity and hepatitis B related acute-on-chronic liver failure. In this study, we developed Taqman amplification refractory mutation system (Taqman-ARMS) and established a strict control system to detect HBV G1896A mutant. HBV viral DNA was isolated from 60 patient serum samples, and full-length HBV genome was cloned. Then, Taqman-ARMS was used to detect HBV G1896A mutant. The assay has the sensitivity of 1E+3 IU/ml G1896A template, and 0.1% weak population virus with G1896A could be found in mixtures. Total of all 60 clinical samples random collected were detected by Taqman-ARMS, the results were consistent with those by DNA sequencing. The proposed Taqman-ARMS real-time PCR method for the detection of G1896A mutation of HBV was rapid, simple, sensitive, specific, and applicable in the clinical setting.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057122111

Download citation: RISBibTeXText

PMID: 25968238

DOI: 10.1002/jcla.21857

Related references

Sensitive and accurate quantitation of hepatitis B virus DNA using a kinetic fluorescence detection system (TaqMan PCR). Journal of Virological Methods 85(1-2): 75-82, 2000

Intrauterine-transplacental transmission of hepatitis B virus (HBV) from hepatitis B e antigen negative (precore mutant, G1896A) chronic HBV infected mothers to their infants. Preliminary results of a prospective study. Journal of Clinical Virology 38(2): 181-183, 2006

Rapid detection of the hepatitis B virus YMDD mutant using TaqMan-minor groove binder probes. Clinica Chimica Acta; International Journal of Clinical Chemistry 395(1-2): 151-154, 2008

Rapid RT-PCR detection of hepatitis G virus RNA in serum using the TaqMan fluorogenic detection system. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 588, 1996

Rapid reverse transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system. Journal of Clinical Microbiology 34(12): 2933-2936, 1996

High-throughput quantitative analysis of hepatitis B virus DNA in serum using the TaqMan fluorogenic detection system. Hepatology 32(3): 626-629, 2000

Comparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis E virus and integration of Feline calicivirus as internal control. Journal of Applied Microbiology 106(4): 1360-1369, 2010

The COBAS ® TaqMan ® hepatitis C virus assays: automated systems for accurate and sensitive viral load quantification. Expert Review of Molecular Diagnostics 11(8): 793-798, 2012

Results of a novel real-time PCR, sequence analysis, Inno-LiPA line probe assays in the detection of hepatitis B virus G1896A precore mutation in French blood donors. Pathologie-Biologie 59(2): E21-E27, 2011

TaqMan real-time reverse transcription-PCR assay for universal detection and quantification of avian hepatitis E virus from clinical samples in the presence of a heterologous internal control RNA. Journal of Clinical Microbiology 49(4): 1339-1346, 2011

Performance of the New Aptima HCV Quant Dx Assay in Comparison to the Cobas TaqMan HCV2 Test for Use with the High Pure System in Detection and Quantification of Hepatitis C Virus RNA in Plasma or Serum. Journal of Clinical Microbiology 54(4): 1101-1107, 2016

Hepatitis B virus genotype B with G1896A and A1762T/G1764A mutations is associated with hepatitis B related acute-on-chronic liver failure. Journal of Medical Virology 83(9): 1544-1550, 2012

Anomalous quantitation standard growth curves in a laboratory-developed hepatitis C virus (HCV) RNA quantification assay using the TaqMan HCV analyte-specific reagent. Journal of Clinical Microbiology 47(7): 2317-2320, 2009

Ultrasensitive amplification refractory mutation system real-time PCR (ARMS RT-PCR) assay for detection of minority hepatitis B virus-resistant strains in the era of personalized medicine. Journal of Clinical Microbiology 51(9): 2893-2900, 2014