+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Analysis of RNA from Alzheimer's Disease Post-mortem Brain Tissues

Analysis of RNA from Alzheimer's Disease Post-mortem Brain Tissues

Molecular Neurobiology 53(2): 1322-1328

Alzheimer's disease (AD) is a uniquely human, age-related central nervous system (CNS) disorder for which there is no adequate experimental model. While well over 100 transgenic murine models of AD (TgAD) have been developed that recapitulate many of the neuropathological features of AD, key pathological features of AD such as progressive neuronal atrophy, neuron cell loss, and neurofibrillary tangle (NFT) formation have not been observed in any TgAD model to date. To more completely analyze and understand the neuropathology, altered neuro-inflammatory and innate-immune signaling pathways, and the complex molecular-genetics and epigenetics of AD, it is therefore necessary to rigorously examine short post-mortem interval (PMI) human brain tissues to gain a deeper and more thorough insight into the neuropathological mechanisms that characterize the AD process. This perspective-methods paper will highlight some important recent findings on the utilization of short PMI tissues in sporadic (idiopathic; of unknown origin) AD research with focus on the extraction and quantification of RNA, and in particular microRNA (miRNA) and messenger RNA (mRNA) and analytical strategies, drawing on the authors' combined 125 years of laboratory experience into this investigative research area. We sincerely hope that new investigators in the field of "gene expression analysis in neurological disease" will benefit from the observations presented here and incorporate these recent findings and observations into their future experimental planning and design.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057190104

Download citation: RISBibTeXText

PMID: 25631714

DOI: 10.1007/s12035-015-9105-6

Related references

Phosphoinositide hydrolysis, G alpha q, phospholipase C, and protein kinase C in post mortem human brain: effects of post mortem interval, subject age, and Alzheimer's disease. Neuroscience 69(1): 125-138, 1995

Expression of interleukin-1 receptor antagonist protein in post-mortem human brain tissues of Alzheimer's disease and control cases. Acta Neuropathologica 93(4): 414-420, 1997

Oxidative Stress Levels in the Brain Are Determined by Post-Mortem Interval and Ante-Mortem Vitamin C State but Not Alzheimer's Disease Status. Nutrients 10(7):, 2018

Network Topology Analysis of Post-Mortem Brain Microarrays Identifies More Alzheimer's Related Genes and MicroRNAs and Points to Novel Routes for Fighting with the Disease. Plos one 11(1): E0144052, 2016

Histological analysis of post mortem Alzheimer's disease and cerebral amyloid angiopathic brain at point of small hypointensities in susceptibility weighted MR images. 2008

Autophagic and lysosomal defects in human tauopathies: analysis of post-mortem brain from patients with familial Alzheimer disease, corticobasal degeneration and progressive supranuclear palsy. Acta Neuropathologica Communications 4: 22, 2016

Evaluation of the Post-Mortem Superoxide Dismutase, Glutathione Peroxidase Activities and Malondialdehyde Level in Renal and Brain Tissues: Is it Possible to Estimate Post-Mortem Interval Using these Parameters?. Clinical Laboratory 61(9): 1205-1211, 2015

Stability of brain Rna post mortem : effect of Alzheimer's disease. Biochemical Society Transactions 15(3): 558-559, 1987

Post mortem sampling of the brain and other tissues in neurodegenerative disease. Histopathology 44(4): 309-317, 2004

N-acetylaspartate and N-acetylaspartylglutamate levels in Alzheimer's disease post-mortem brain tissue. Journal of the Neurological Sciences 127(2): 230-233, 1994

ZnT3 mRNA levels are reduced in Alzheimer's disease post-mortem brain. Molecular Neurodegeneration 4: 53, 2009

Increased acetyl and total histone levels in post-mortem Alzheimer's disease brain. Neurobiology of Disease 74: 281-294, 2015

From brain to food: analysis of phosphatidylcholins, lyso-phosphatidylcholins and phosphatidylcholin-plasmalogens derivates in Alzheimer's disease human post mortem brains and mice model via mass spectrometry. Journal of Chromatography. a 1218(42): 7713-7722, 2011

16S rRNA Next Generation Sequencing Analysis Shows Bacteria in Alzheimer's Post-Mortem Brain. Frontiers in Aging Neuroscience 9: 195, 2017

Post-mortem analysis of neuroinflammatory changes in human Alzheimer's disease. Alzheimer's Research and Therapy 7(1): 42, 2015