+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease



Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease



Biomedical Papers of the Medical Faculty of the University Palacky Olomouc Czechoslovakia 160(2): 271-275



To assess the impact of autologous serum eye drops on the level of ocular surface apoptosis in patients with bilateral severe dry eye disease. This prospective study was conducted on 10 patients with severe dry eye due to graft versus host disease (group 1) and 6 patients with severe dry eye due to primary Sjögren's syndrome (group 2). Impression cytology specimens from the bulbar conjunctiva were obtained before and after a three-month treatment with 20% autologous serum eye drops applied a maximum of 12 times a day together with regular therapy with artificial tears. The percentage of apoptotic epithelial cells was evaluated immunochemically using anti-active caspase 3 antibody. In group 1, the mean percentage of apoptotic cells was 3.6% before the treatment. The three-month treatment led to a significant decrease to a mean percentage of 1.8% (P = 0.028). The mean percentage of apoptotic conjunctival cells decreased from 5.4% before the treatment to 3.8% in group 2; however, these results did not reach the level of significance. Three-month autologous serum treatment led to the improvement of ocular surface apoptosis, especially in the group of patients with severe dry eye due to graft versus host disease. This result supports the very positive effect of autologous serum on the ocular surface in patients suffering from severe dry eye.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 057219871

Download citation: RISBibTeXText

PMID: 26927466


Related references

The application of the autologous serum eye drops results in significant improvement of the conjunctival status in patients with the dry eye syndrome. Ceska a Slovenska Oftalmologie 64(2): 52-56, 2008

The application of autologous serum eye drops in severe dry eye patients; subjective and objective parameters before and after treatment. Current Eye Research 39(1): 21-30, 2014

Combined application of autologous serum eye drops and silicone hydrogel lenses for the treatment of persistent epithelial defects. Eye and Contact Lens 37(6): 370-373, 2011

Autologous serum eye drops for the treatment of severe dry eye in patients with chronic graft-versus-host disease. Bone Marrow Transplantation 31(7): 579-583, 2003

The effect of autologous serum eye drops in the treatment of severe dry eye disease: a prospective randomized case-control study. American Journal of Ophthalmology 140(3): 565; Author Reply 565-6, 2005

Effects of Components of Serum on Apoptosis in Cultured Conjunctival Epithelial Cells. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 3171, 2002

Coculture of autologous limbal and conjunctival epithelial cells to treat severe ocular surface disorders: long-term survival analysis. Indian Journal of Ophthalmology 61(5): 202-207, 2013

Autologous serum eye drops for severe dry eye syndrome in a patient with chronic graft-versus-host disease: a case report. International Journal of Pharmaceutical Compounding 18(5): 370-377, 2015

Elucidation of apoptosis induced by serum deprivation in cultured conjunctival epithelial cells. British Journal of Ophthalmology 90(6): 760-764, 2006

Effects of Autologous Serum Eye Drops on Conjunctival Expression of MUC5AC in Patients With Ocular Surface Disorders. Cornea 35(3): 336-341, 2016

Effect of D-3-Hydroxybutyrate on Apoptosis Induced by Serum Deprivation in Cultured Conjunctival Epithelial Cells. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 3161, 2002

Treatment of severe chronic ocular graft-versus-host disease using 100% autologous serum eye drops from a sealed manufacturing system: a retrospective cohort study. British Journal of Ophthalmology 101(3): 322-326, 2017

Autologous Serum Eye Drops Accelerate Epithelial Healing After LASEK. Current Eye Research 41(6): 873, 2016

Autologous Serum Eye Drops Accelerate Epithelial Healing After LASEK. Current Eye Research 41(1): 15-19, 2016

Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: a case report of unique application. Cornea 22(5): 478-481, 2003