+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Crosslinking induces high mineralization of apatite minerals on collagen fibers

Crosslinking induces high mineralization of apatite minerals on collagen fibers

International Journal of Biological Macromolecules 113: 450-457

Type I collagen, as the critical organic component of bone matrix, plays a template role in mineralization. Gamma-ray irradiation is used to prepare collagen with different crosslinking degrees without introducing foreign substances for mineralization. Significant improvement in crosslinking degree of collagen can be inferred by enzymolysis and differential scanning calorimetry analysis. Scanning electron microscopy observation reveals that, as crosslinking degree increases, the pore structure of collagen becomes more compact. Fourier transform infrared spectroscopy analysis shows the intact retention of classical triple-helical structure of collagen after crosslinking and the following mineralization. Atomic force microscopy characterization, calcium and phosphorus assay kit tests and thermogravimetry measurements indicate that the more compact the collagen structure, the more hydroxyapatite can attach on collagen fibers. These findings reveal the high crosslinking degree of collagen is favorable for the improvement of mineralization ability by providing a more stable and compact network structure. Such crosslinked collagen may provide a more potential way for the preparation of bone substitute materials.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057279907

Download citation: RISBibTeXText

PMID: 29481955

DOI: 10.1016/j.ijbiomac.2018.02.136

Related references

Is the absence of mineralization or incomplete mineralization in the scales of osteichthyes connected with the typical spatial organization of collagen fibers?. Bulletin de la Societe Zoologique de France 106(3): 371-372, 1981

The effects of prestrain and collagen fibril alignment on in vitro mineralization of self-assembled collagen fibers. Connective Tissue Research 46(2): 107-115, 2005

Physical and specific crosslinking of collagen fibers by supramolecular nanogelators. Advanced Materials 23(26): 2957-2961, 2011

Mechanochemical melting of collagen fibers. 3. Cooperativity induced by crosslinking. Biopolymers 8(6): 787-794, 1969

Reversible crosslinking of bovine collagen fibers with cystine methylester. New strategies for improving animal production for human welfare: proceedings the Fifth World Conference on Animal Production August 14-19-1983: 750, 1983

Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering. Biomaterials 104: 323-338, 2017

Electrospun nanostructured fibers of collagen-biomimetic apatite on titanium alloy. Bioinorganic Chemistry and Applications 2012: 123953, 2012

Structure and dynamics of water in native and tanned collagen fibers: Effect of crosslinking. International Journal of Biological Macromolecules 47(5): 590-596, 2011

Mechano chemical melting of collagen fibers part 3 cooperativity induced by crosslinking. Biopolymers: 787-794, 1969

Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment. Journal of Biomedical Materials Research 29(11): 1373-1379, 1995

Water-stable electrospun collagen fibers from a non-toxic solvent and crosslinking system. Journal of Biomedical Materials Research. Part a 101(5): 1237-1247, 2013

Degree of mineralization-related collagen crosslinking in the femoral neck cancellous bone in cases of hip fracture and controls. Calcified Tissue International 79(3): 160-168, 2006

Calcification. XXIV. Mineralization of collagen and other fibers. Archives of Biochemistry and Biophysics 84: 79-95, 1959

Spatial organization of collagen fibers and mineralization in the scales of osteichthyes. Bulletin de la Societe Zoologique de France 106(3): 370-371, 1981

Efficacy and safety of transepithelial corneal collagen crosslinking surgery versus standard corneal collagen crosslinking surgery for keratoconus: a meta-analysis of randomized controlled trials. Bmc Ophthalmology 17(1): 262, 2018