+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Brugia malayi infective larvae fail to activate Langerhans cells and dermal dendritic cells in human skin

Brugia malayi infective larvae fail to activate Langerhans cells and dermal dendritic cells in human skin

Parasite Immunology 37(2): 79-91

Filarial infection in humans is initiated when a mosquito deposits third-stage parasite larvae (L3) in the skin. Langerhans cells (LCs) and dermal dendritic cells (DDCs) are the first cells that the parasite encounters, and L3s must evade these highly effective antigen-presenting cells to establish infection. To assess LC and DDC responses to L3 in human skin, we employed three models of increasing physiologic relevance: in vitro-generated LCs, epidermal blister explants and full-thickness human skin sections. In vitro-generated LCs expressed TLR1-10 and robustly produced IL-6 and TNF-α in response to PolyI:C, but pre-exposure to L3s did not alter inflammatory cytokine production or TLR expression. L3s did not modulate expression of LC markers CDH1, CD207, or CD1a, or the regulatory products TSLP or IDO in epidermal explants or in vitro-generated LC. LC, CD14+ DDC, CD1c+ DC and CD141+ DC from human skin sections were analysed by flow cytometry. While PolyI:C potently induced CCL22 production in LC, CD1c+ DC, and CD141+ DC, and IL-10 production in LC, L3s did not modulate the numbers of or cytokine production by any skin DC subset. L3s broadly failed to activate or modulate LCs or DDCs, suggesting filarial larvae expertly evade APC detection in human skin.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057329225

Download citation: RISBibTeXText

PMID: 25545218

DOI: 10.1111/pim.12169

Related references

Brugia malayi microfilariae induce cell death in human dendritic cells, inhibit their ability to make IL-12 and IL-10, and reduce their capacity to activate CD4+ T cells. Journal of Immunology 171(4): 1950-1960, 2003

Immunohistochemical study of epidermal Langerhans cells and dermal dendritic cells in benign and malignant skin lesions characterized by a dermal lymphoid infiltrate consisting either of B-cells or T-cells. Virchows Archiv. A Pathological Anatomy and Histopathology 411(4): 337-343, 1987

Antibody-mediated cytotoxic effects in vitro and in vivo of rat cells on infective larvae of Brugia malayi. International Journal for Parasitology 20(6): 725-730, 1990

Relay of herpes simplex virus between Langerhans cells and dermal dendritic cells in human skin. Plos Pathogens 11(4): E1004812, 2015

Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin. Journal of Immunology 168(9): 4361-4371, 2002

Epidermal Langerhans' cells and dermal dendritic cells in human fetal and neonatal skin: an immunohistochemical study. Pediatric Dermatology 4(1): 11-17, 1987

Effects of solar ultraviolet radiation on engineered human skin equivalent containing both Langerhans cells and dermal dendritic cells. Tissue Engineering 13(11): 2667-2679, 2007

Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions. Journal of Leukocyte Biology 84(1): 143-151, 2008

Epitopes for CD1a, CD1b, and CD1c antigens are differentially mapped on Langerhans cells, dermal dendritic cells, keratinocytes, and basement membrane zone in human skin. Journal of the American Academy of Dermatology 27(3): 419-426, 1992

Langerhans cells and dermal dendritic cells capture protein antigens in the skin: possible targets for vaccination through the skin. Immunobiology 215(9-10): 770-779, 2011

Langerhans cells, dermal dendritic cells and monocyte-derived dendritic cells are distinct subsets of myeloid dendritic cells defined by diverse functional properties. Archives of Dermatological Research 295(8-9): 342, 2004

In vitro cultivation of Brugia malayi and Brugia pahangi infective larvae to fourth and fifth stage. Transactions of the Royal Society of Tropical Medicine and Hygiene6(5): 702-703, 1982

Scanning electron microscopic study of subperiodic Brugia malayi and Brugia pahangi infective larvae (Filarioidea: Onchocercidae). Tropical Biomedicine 1(1): 29-35, 1984

The response of human macrophages and dendritic cells to live microfilariae of Brugia malayi. American Journal of Tropical Medicine & Hygiene 69(3 Suppl.): 567, 2003

Migration of Langerhans cells and dermal dendritic cells in skin organ cultures: augmentation by Tnf- and Il-1. Journal of Leukocyte Biology 66(3): 462-470, 1999