+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Carcinoembryonic antigen detection with "Handing"-controlled fluorescence spectroscopy using a color matrix for point-of-care applications

Carcinoembryonic antigen detection with "Handing"-controlled fluorescence spectroscopy using a color matrix for point-of-care applications

Biosensors and Bioelectronics 90: 508-515

In this study, we developed a power-free, accurate, and portable diagnostic platform called Handing based on embedded technology, which can rapidly detect fluorescent signals from quantum dots (QDs) on lateral flow test strips (LFTSs). The Handing system has three components: a hand-held test terminal, LFTS cartridge, and data server. The hand-held test terminal is the primary system component and it is integrated with tiny components using printed circuit board packaging for image acquisition, processing, and data handling by a specific program. A black smooth shell and three-dimensional printed test cartridge are used to facilitate the portability of the detection terminal, which provides a closed detection process as well as enhancing the anti-interference capacity. The functions of the data server comprise pre-editing, storage, range querying, and sharing with an international network. Multiple hand-held terminal devices can be linked simultaneously to the same data server. In this study, we detected the tumor marker carcinoembryonic antigen (CEA) using the QD-LFTS system, which allowed quantitative analysis in the range of 1-100ng/mL with an ideal detection limit of 0.049ng/mL. Thus, the system is suitable for detecting CEA in the clinically accepted range. We also detected 70 positive and 30 negative serum samples using the Handing system, which exhibited good specificity and sensitivity. Thus, Handing has the capacity for rapid quantitative detection with high stability and repeatability, so it could be used for in vitro diagnostics in the laboratory and in other conditions for various applications, e.g., food evaluation, disease screening, environmental monitoring, and drug testing.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057354690

Download citation: RISBibTeXText

PMID: 27825889

DOI: 10.1016/j.bios.2016.10.052

Related references

Bioresponsive Release System for Visual Fluorescence Detection of Carcinoembryonic Antigen from Mesoporous Silica Nanocontainers Mediated Optical Color on Quantum Dot-Enzyme-Impregnated Paper. Analytical Chemistry 89(9): 5152-5160, 2017

Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications. Biomedical Optics Express 4(7): 1074-1082, 2013

A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications. Biosensors and Bioelectronics 117: 153-160, 2018

Two-point separation in far-field super-resolution fluorescence microscopy based on two-color fluorescence dip spectroscopy, Part I: Experimental evaluation. Applied Spectroscopy 59(7): 868-872, 2005

The application of CdTe/CdS in the detection of carcinoembryonic antigen by fluorescence polarization immunoassay. Journal of Fluorescence 22(6): 1571-1579, 2012

Immunological heterogeneity of carcinoembryonic antigen: immunohistochemical detection of carcinoembryonic antigen determinants in colonic tumors with monoclonal antibodies. Cancer Research 43(2): 693-701, 1983

Plasma-enhanced antibody immobilization for the development of a capillary-based carcinoembryonic antigen immunosensor using laser-induced fluorescence spectroscopy. Analytical Chemistry 85(9): 4578-4585, 2013

Compact point-detection fluorescence spectroscopy system for quantifying intrinsic fluorescence redox ratio in brain cancer diagnostics. Journal of Biomedical Optics 16(3): 037004, 2011

Picomolar detection of carcinoembryonic antigen in whole blood using microfluidics and surface-enhanced Raman spectroscopy. Electrophoresis 37(5-6): 786-789, 2016

Specific detection of carcinoembryonic antigen based on fluorescence quenching of Au-Ag core-shell nanotriangle probe. Sensors and Actuators B: Chemical 233: 214-222, 2016

Carcinoembryonic antigen in tissue and serum clinical applications in prognosis and early detection of relapse. Breast Cancer Research & Treatment 14(1): 169, 1989

One-step separation-free detection of carcinoembryonic antigen in whole serum: Combination of two-photon excitation fluorescence and optical trapping. Biosensors and Bioelectronics 90: 146-152, 2017

Specific Detection of Carcinoembryonic Antigen Based on Fluorescence Quenching of Hollow Porous Gold Nanoshells with Roughened Surface. Acs Applied Materials and Interfaces 9(42): 36632-36641, 2017

Applications of dual-color fluorescence cross-correlation spectroscopy in antibody binding studies. Analytical Biochemistry 374(1): 182-195, 2008

An origami electrochemiluminescence immunosensor based on gold/graphene for specific, sensitive point-of-care testing of carcinoembryonic antigen. Sensors and Actuators B: Chemical 193: 247-254, 2014