+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus



Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus



Bmc Plant Biology 15: 27



Carotenoids are indispensable plant secondary metabolites that are involved in photosynthesis, antioxidation, and phytohormone biosynthesis. Carotenoids are likely involved in other biological functions that have yet to be discovered. In this study, we integrated genomic, biochemical, and cellular studies to gain deep insight into carotenoid-related biological processes in citrus calli overexpressing CrtB (phytoene synthase from Pantoea agglomerans). Fortunella hindsii Swingle (a citrus relative) and Malus hupehensis (a wild apple) calli were also utilized as supporting systems to investigate the effect of altered carotenoid accumulation on carotenoid-related biological processes. Transcriptomic analysis provided deep insight into the carotenoid-related biological processes of redox status, starch metabolism, and flavonoid/anthocyanin accumulation. By applying biochemical and cytological analyses, we determined that the altered redox status was associated with variations in O2 (-) and H2O2 levels. We also ascertained a decline in starch accumulation in carotenoid-rich calli. Furthermore, via an extensive cellular investigation of the newly constructed CrtB overexpressing Fortunella hindsii Swingle, we demonstrated that starch level reducation occurred in parallel with significant carotenoid accumulation. Moreover, studying anthocyanin-rich Malus hupehensis calli showed a negative effect of carotenoids on anthocyanin accumulation. In citrus, altered carotenoid accumulation resulted in dramatic effects on metabolic processes involved in redox modification, starch degradation, and flavonoid/anthocyanin biosynthesis. These findings provided new perspectives to understand the biological importance of carotenogenesis and of the developmental processes associated with the nutritional and sensory qualities of agricultural products that accumulate carotenoids.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057360701

Download citation: RISBibTeXText

PMID: 25644332

DOI: 10.1186/s12870-015-0426-4


Related references

Flavonoid metabolism in relation to differential anthocyanin accumulation in juvenile and mature phase hedera helix l. Hortscience 23(3 SECT 2): 733, 1988

Distinct Carotenoid and Flavonoid Accumulation in a Spontaneous Mutant of Ponkan (Citrus reticulata Blanco) Results in Yellowish Fruit and Enhanced Postharvest Resistance. Journal of Agricultural and Food Chemistry 63(38): 8601-8614, 2016

Effects of fruit bagging on accumulation of anthocyanin, flavonoid, and simple phenols at harvest and on anthocyanin synthesis potential during storage in Delicious apples. Hortscience 33(3): 497, 1998

The influence of ascorbate on anthocyanin accumulation during high light acclimation in Arabidopsis thaliana: further evidence for redox control of anthocyanin synthesis. Plant, Cell and Environment 35(2): 388-404, 2014

Diversity in the carotenoid profiles and the expression of genes related to carotenoid accumulation among citrus genotypes. Breeding Science 66(1): 139-147, 2016

Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro. Journal of Experimental Botany 63(2): 871-886, 2014

Gene expression in flavonoid biosynthesis: correlation with flavonoid accumulation in developing citrus fruit. Physiologia Plantarum 111(1): 66-74, 2001

Transcriptomic changes triggered by carotenoid biosynthesis inhibitors and role of Citrus sinensis phosphate transporter 4;2 (CsPHT4;2) in enhancing carotenoid accumulation. Planta 2018, 2018

Effect of postharvest ethylene treatment on carotenoid accumulation and the expression of carotenoid biosynthetic genes in the flavedo of orange (Citrus sinensis L. Osbeck) fruit. Postharvest Biology and Technology 43(1): 14-22, 2007

Chlorophyll, Carotenoid and Anthocyanin Accumulation in Mung Bean Seedling Under Clinorotation. Microgravity Science and Technology 29(6): 427-432, 2017

Effect of calcium on strawberry fruit flavonoid pathway gene expression and anthocyanin accumulation. Plant Physiology and Biochemistry 82: 289-298, 2015

Mechanism of Carotenoid Accumulation in Citrus Fruit. Journal of the Japanese Society for Horticultural Science 81(3): 219-233, 2012

Expression of multi-copy flavonoid pathway genes coincides with anthocyanin, flavonol and flavan-3-ol accumulation of grapevine. Vitis 47(3): 135-140, 2008

Synergistic metabolism in hybrid corn indicates bottlenecks in the carotenoid pathway and leads to the accumulation of extraordinary levels of the nutritionally important carotenoid zeaxanthin. Plant Biotechnology Journal 9(3): 384-393, 2011

Atlas of rice grain filling-related metabolism under high temperature: joint analysis of metabolome and transcriptome demonstrated inhibition of starch accumulation and induction of amino acid accumulation. Plant and Cell Physiology 51(5): 795-809, 2010