+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization and cloning of the cry2A gene from indigenous isolates of Bacillus thuringiensis



Characterization and cloning of the cry2A gene from indigenous isolates of Bacillus thuringiensis



Molekuliarnaia Biologiia 49(4): 585-591



Discovery of novel cry genes of Bacillus thuringiensis (Bt) with higher toxicity is important for the development of transgenic Bt crops resistant to target pests. Two new indigenous isolates of Bt were characterized for their colony type, crystal inclusion and toxicity with Helicoverpa armigera Hubner and Spodoptera litura Linn. Screening of cry2A genes from the two new isolates of Bt, T30 and T48, by PCR with cry2A family primers showed the presence of cry2A genes only in the isolate T30. Further screening of these isolates of Bt with three different cry2A gene specific primers showed the presence of cry2Aa, cry2Ab and cry2Ac genes in both of the new Bt isolates. The cry2A open reading frame (ORF) of Bt T48 (~1.9 kb) was amplified and cloned in a T/A vector (~2.8 kb). All the E. coli transformants showed only the cry2Aa gene. Comparison of the nucleotide sequence data generated from the cry2Aa (~1.9 kb) gene showed a 99 percent homology and one amino acid variation when compared with the holotype sequence of Cry2Aa1.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 057385732

Download citation: RISBibTeXText

PMID: 26299858


Related references

Analysis, of Cry2A proteins encoded by genes cloned from indigenous isolates of Bacillus thuringiensis for toxicity against Helicoverpa armigera. Current Science 86(4): 566-570, 2004

Cloning, characterization and expression of a novel haplotype cry2A-type gene from Bacillus thuringiensis strain SWK1, native to Himalayan valley Kashmir. Journal of Invertebrate Pathology 136: 1-6, 2016

Cloning, characterization, and expression of a new cry1Ab gene from DOR Bt-1, an indigenous isolate of Bacillus thuringiensis. Molecular Biotechnology 54(3): 795-802, 2013

Cloning and characterization of truncated cry1Ab gene from a new indigenous isolate of Bacillus thuringiensis. Biotechnology Letters 32(9): 1311-1315, 2010

Characterization of a cry2A gene cloned from an isolate of Bacillus thuringiensis serovar sotto. Current microbiology 35(1): 1-8, 1997

Characterization of a cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic larvicidal activity of its encoded protein with Cry2A delta-endotoxin of B. thuringiensis kurstaki on Culex pipiens (common house mosquito). Biotechnology and Applied Biochemistry 44(Pt 1): 19-25, 2006

Characterization of a cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic larvicidal activity of its encoded protein with Cry2A delta -endotoxin of B. thuringiensis kurstaki on Culex pipiens (common house mosquito). Biotechnology and Applied Biochemistry 44(1): 19-25, 2006

Differential effects of helper proteins encoded by the cry2A and cry11A operons on the formation of Cry2A inclusions in Bacillus thuringiensis. Fems Microbiology Letters 165(1): 35-41, 1998

Characterization and evaluation of two indigenous Bacillus thuringiensis isolates against Helicoverpa armigera Hubner. 2012

Cloning of cry2Aa gene from an indigenous isolate of Bacillus thuringiensis. Madras Agricultural Journal 88(4/6): 198-202, 2001

Cloning of cry2Aa and cry2Ab genes from new isolates of Bacillus thuringiensis and their expression in recombinant Bacillus thuringiensis and Escherichia coli strains. World Journal of Microbiology and Biotechnology 20(1): 11-17, 2004

Insecticidal toxins from bacillus thuringiensis ssp kenyae gene cloning and characterization and comparison with bacillus thuringiensis ssp kurstaki cry i ac toxins. Applied & Environmental Microbiology 57(2): 349-358, 1991

Cloning and characterization of a Bacillus thuringiensis homolog of the spoIIID gene from Bacillus subtilis. Gene 154(1): 23-29, 1995

Characterization of the cry1Ac17 gene from an indigenous strain of Bacillus thuringiensis subsp. kenyae. Current Microbiology 57(6): 570-574, 2008