+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of Two Monoclonal Antibodies That Recognize Linker Region and Carboxyl Terminal Domain of Coronavirus Nucleocapsid Protein



Characterization of Two Monoclonal Antibodies That Recognize Linker Region and Carboxyl Terminal Domain of Coronavirus Nucleocapsid Protein



Plos one 11(9): E0163920



The transmissible gastroenteritis virus (TGEV) nucleocapsid (N) protein plays important roles in the replication and translation of viral RNA. The present study provides the first description of two monoclonal antibodies (mAbs) (5E8 and 3D7) directed against the TGEV N protein linker region (LKR) and carboxyl terminal domain (CTD). The mAbs 5E8 and 3D7 reacted with native N protein in western blotting and immunofluorescence assay (IFA). Two linear epitopes, 189SVEQAVLAALKKLG202 and 246VTRFYGARSSSA257, located in the LKR and CTD of TGEV N protein, respectively, were identified after truncating the protein and applying a peptide scanning technique. Using mAb 5E8, we observed that the N protein was expressed in the cytoplasm during TGEV replication and that the protein could be immunoprecipitated from TGEV-infected PK-15 cells. The mAb 5E8 can be applied for different approaches to diagnosis of TGEV infection. In addition, the antibodies represent useful tools for investigating the antigenic properties of the N protein.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 057387554

Download citation: RISBibTeXText

PMID: 27689694


Related references

Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein. Febs Letters 587(2): 120-127, 2013

Preparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein. Di 1 Jun Yi Da Xue Xue Bao 24(1): 1-6, 2004

Preparation and characterization of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein. Chinese Journal of Experimental and Clinical Virology 18(4): 316-320, 2004

Preparation and characterization of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein. Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 18(4): 316-320, 2004

Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Japanese Journal of Veterinary Research 55(4): 115-127, 2008

Development, characterization, and application of monoclonal antibodies against severe acute respiratory syndrome coronavirus nucleocapsid protein. Clinical and Vaccine Immunology 17(12): 2033-2036, 2010

Monoclonal antibodies against the minimal DNA-binding domain in the carboxyl-terminal region of human immunodeficiency virus type 1 integrase. Journal of Virology 73(5): 4475-4480, 1999

Identification of functional regions in the carboxyl terminal domain of escherichia coli ribosomal protein s1 using monoclonal antibodies. Biochemical & Biophysical Research Communications 149(1): 34-39, 1987

Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. American Journal of Pathology 148(5): 1543-1555, 1996

Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Applied Microbiology and Biotechnology 100(1): 181-192, 2016

Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target. Journal of Medicinal Chemistry 57(6): 2247-2257, 2014

A major determinant for membrane protein interaction localizes to the carboxy-terminal domain of the mouse coronavirus nucleocapsid protein. Journal of Virology 79(21): 13285-13297, 2005

SARS coronavirus nucleocapsid protein monoclonal antibodies developed using a prokaryotic expressed protein. Hybridoma 30(5): 481-485, 2011

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of human coronavirus OC43 nucleocapsid protein. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 66(Pt 7): 815-818, 2010

Crystallographic analysis of the N-terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein. Acta Crystallographica. Section F Structural Biology Communications 71(Pt 8): 977-980, 2015