+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chemical and Genetic Comparative Analysis of Gentiana crassicaulis and Gentiana macrophylla



Chemical and Genetic Comparative Analysis of Gentiana crassicaulis and Gentiana macrophylla



Chemistry and Biodiversity 13(6): 776-781



Gentiana crassicaulis Duthie ex Burk. and Gentiana macrophylla Pall. are two main sources of Radix Gentianae Macrophyllae (Qinjiao) available in markets, which has a wide range of anti-inflammatory effects and has been extensively used for fighting rheumatoid arthritis. However, they vary in terms of chemical compositions, pharmacological activities, and biomass. In this study, a combined chemical and genetic (HPLC and DNA barcoding) approach was used to compare these two plants. Four predominant bioactive compounds, namely, gentiopicroside, loganic acid, swertiamarin, and sweroside, were used to assess the chemical variations. Based on chemical variations, 15 samples were clustered into two groups through PCA analyses. DNA barcoding utilizing the variable nuclear ITS2 regions were sequenced, aligned, and compared. Together with 61 sequences collected from GenBank, 76 batches of Qinjiao were clustered in two groups according to species origin. The genetic relationships indicated by the ITS2-based NJ tree were consistent with the chemical variations. Thus, the chemical profiles determined by HPLC and DNA profiles obtained from ITS2 region could be applied for the quality control of Qinjiao.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057393862

Download citation: RISBibTeXText

PMID: 27144464

DOI: 10.1002/cbdv.201500247


Related references

On various xanthones of gentiana lutea gentiana purpurea gentiana punctata gentiana pannonica. Annales Pharmaceutiques Francaises 31(6):, 1973

Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents. Journal of Ethnopharmacology 144(3): 638-645, 2012

Comparative analysis among three Taiwan-specific Gentiana species and Chinese medicinal plant Gentiana scabra. Botanical Studies 54(1): 54, 2013

The chemical constituents from the roots of Gentiana crassicaulis and their inhibitory effects on inflammatory mediators NO and TNF-?. Natural Products and Bioprospecting 2(5): 217-221, 2012

Chemical constituents of Gentiana macrophylla Pall. Natural Product Research 24(14): 1365-1369, 2010

Comparative study on trace elements in Gentiana macrophlla and Gentiana officinalis from Gansu. Chinese Journal Of Information On Traditional Chinese Medicine: 2, 34-35, 2008

Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi. Zhongguo Zhong Yao Za Zhi 30(19): 1519-1522, 2005

Investigation of the Chemical Constituents of Gentiana Macrophylla. Ii. the Structure and Synthesis of Gentianidine. Yao Xue Xue Bao 11: 412-416, 1964

Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats. Zhong Yao Cai 34(4): 517-519, 2011

Optimum harvest study of Gentiana crassicaulis in Ludian. Zhongguo Zhong Yao Za Zhi 39(14): 2635-2639, 2014

Fertilizer Effect on Yield and Quality of Gentiana crassicaulis. Zhong Yao Cai 38(9): 1798-1803, 2015

Development of ten microsatellite loci for Gentiana crassicaulis (Gentianaceae). Conservation genetics 8(6): 1499-1501, 2007

Study on seed biological characteristics of Gentiana crassicaulis. Zhongguo Zhong Yao Za Zhi 36(5): 552-555, 2011

Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis. Carbohydrate Polymers 172: 306-314, 2017

Effect of P and K Fertilization on Four Iridoids in Axial and Lateral Root of Gentiana crassicaulis. Zhong Yao Cai 39(2): 240-244, 2016