+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cloud Point and Liquid-Liquid Equilibrium Behavior of Thermosensitive Polymer L61 and Salt Aqueous Two-Phase System

Cloud Point and Liquid-Liquid Equilibrium Behavior of Thermosensitive Polymer L61 and Salt Aqueous Two-Phase System

Journal of Physical Chemistry. B 119(25): 8201-8208

The cloud point of thermosensitive triblock polymer L61, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO), was determined in the presence of various electrolytes (K2HPO4, (NH4)3C6H5O7, and K3C6H5O7). The cloud point of L61 was lowered by the addition of electrolytes, and the cloud point of L61 decreased linearly with increasing electrolyte concentration. The efficacy of electrolytes on reducing cloud point followed the order: K3C6H5O7 > (NH4)3C6H5O7 > K2HPO4. With the increase in salt concentration, aqueous two-phase systems exhibited a phase inversion. In addition, increasing the temperature reduced the concentration of salt needed that could promote phase inversion. The phase diagrams and liquid-liquid equilibrium data of the L61-K2HPO4/(NH4)3C6H5O7/K3C6H5O7 aqueous two-phase systems (before the phase inversion but also after phase inversion) were determined at T = (25, 30, and 35) °C. Phase diagrams of aqueous two-phase systems were fitted to a four-parameter empirical nonlinear expression. Moreover, the slopes of the tie-lines and the area of two-phase region in the diagram have a tendency to rise with increasing temperature. The capacity of different salts to induce aqueous two-phase system formation was the same order as the ability of salts to reduce the cloud point.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057431116

Download citation: RISBibTeXText

PMID: 26018434

DOI: 10.1021/acs.jpcb.5b03201

Related references

Cloud-point temperature and liquid-liquid phase separation of supersaturated lysozyme solution. Biophysical Chemistry 109(1): 105-112, 2004

Separation, concentration and determination of chloramphenicol in environment and food using an ionic liquid/salt aqueous two-phase flotation system coupled with high-performance liquid chromatography. Analytica Chimica Acta 685(2): 138-145, 2010

Liquid liquid phase equilibrium in the globulin salt water systems vicilin. Journal of the Science of Food & Agriculture 54(2): 239-244, 1991

Liquid liquid phase equilibrium in globulin salt water systems legumin. Journal of the Science of Food & Agriculture 51(3): 345-354, 1990

Separation of curcuminoids using ionic liquid based aqueous two-phase system coupled with in situ dispersive liquid-liquid microextraction. Talanta 149: 6-12, 2016

Protein phase behavior in aqueous solutions: crystallization, liquid-liquid phase separation, gels, and aggregates. Biophysical Journal 94(2): 570-583, 2007

A new dispersive liquid-liquid microextraction using ionic liquid based microemulsion coupled with cloud point extraction for determination of copper in serum and water samples. Ecotoxicology and Environmental Safety 126: 186-192, 2016

PEG-salt aqueous two-phase systems: an attractive and versatile liquid-liquid extraction technology for the downstream processing of proteins and enzymes. Applied Microbiology and Biotechnology 99(16): 6599-6616, 2016

Bulk and dispersed aqueous phase behavior of phytantriol: effect of vitamin E acetate and F127 polymer on liquid crystal nanostructure. Langmuir 22(23): 9512-9518, 2006

Direct automatic determination of polyphenols in olive oils in the aqueous phase of a flow injection liquid liquid extraction system without phase separation. Analytica Chimica Acta 235(2): 441-444, 1990

Ionic liquid-salt aqueous two-phase extraction based on salting-out coupled with high-performance liquid chromatography for the determination of sulfonamides in water and food. Analytical and Bioanalytical Chemistry 405(4): 1245-1255, 2013

Use of the electrically driven emulsion-phase contactor for a biphasic liquid-liquid enzyme system The oxidation of p-cresol by aqueous phase-horseradish peroxidase scientific note. Applied Biochemistry & Biotechnology 51-52(0): 283-289, 1995

Cloud point extraction for high-performance liquid chromatographic speciation of Cr and Cr in aqueous solutions. Journal of Chromatography A 1036(2): 183-188, May 21, 2004

Cloud point extraction for high-performance liquid chromatographic speciation of Cr(III) and Cr(VI) in aqueous solutions. Journal of Chromatography. A 1036(2): 183-188, 2004