+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Colorimetric aptasensor for progesterone detection based on surfactant-induced aggregation of gold nanoparticles



Colorimetric aptasensor for progesterone detection based on surfactant-induced aggregation of gold nanoparticles



Analytical Biochemistry 514: 2-7



This paper proposes an aptasensor for progesterone (P4) detection in human serum and urine based on the aggregating behavior of gold nanoparticles (AuNPs) controlled by the interactions among P4-binding aptamer, target P4 and cationic surfactant hexadecyltrimethylammonium bromide (CTAB). The aptamer can form an aptamer-P4 complex with P4, leaving CTAB free to aggregate AuNPs in this aptasensor. Thus, the sensing solution will turn from red (520 nm) to blue (650 nm) in the presence of P4 because P4 aptamers are used up firstly owing to the formation of an aptamer-P4 complex, leaving CTAB free to aggregate AuNPs. However, in the absence of P4, CTAB combines with aptamers so that AuNPs still remain dispersed. Therefore, this assay makes it possible to detect P4 not only by absorbance measurement but also through naked eyes. By monitoring the variation of absorbance and color, a CTAB-induced colorimetric assay for P4 detection was established with a detection limit of 0.89 nM. Besides, the absorbance ratio A650/A520 has a linear correlation with the P4 concentration of 0.89-500 nM. Due to the excellent recoveries in serum and urine, this biosensor has great potential with respect to the visual and instrumental detection of P4 in biological fluids.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057441074

Download citation: RISBibTeXText

PMID: 27615801

DOI: 10.1016/j.ab.2016.09.006


Related references

Visual detection of cancer cells by colorimetric aptasensor based on aggregation of gold nanoparticles induced by DNA hybridization. Analytica Chimica Acta 904: 92-97, 2016

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles. Microchimica Acta 183(7): 2251-2258, 2016

A label-free colorimetric aptasensor for simple, sensitive and selective detection of Pt (II) based on platinum (II)-oligonucleotide coordination induced gold nanoparticles aggregation. Biosensors and Bioelectronics 85: 771-776, 2017

Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles. Mikrochimica Acta 185(10): 488, 2018

Novel Colorimetric Aptasensor for Zearalenone Detection Based on Nontarget-Induced Aptamer Walker, Gold Nanoparticles, and Exonuclease-Assisted Recycling Amplification. Acs Applied Materials and Interfaces 10(15): 12504-12509, 2018

Magnetic Nanoparticles-based Aptasensor Using Gold Nanoparticles as Colorimetric Probes for the Detection of Salmonella typhimurium. Analytical Sciences 32(4): 431-436, 2016

A novel aptasensor for the colorimetric detection of S. typhimurium based on gold nanoparticles. International Journal of Food Microbiology 245: 1-5, 2017

Development of gold nanoparticles-based aptasensor for the colorimetric detection of organophosphorus pesticide phorate. Analytical and Bioanalytical Chemistry 408(1): 333-338, 2016

Colorimetric detection of mercury(II) based on 2,2-bipyridyl induced quasi-linear aggregation of gold nanoparticles. Sensors and Actuators B: Chemical 215: 421-427, 2015

A simple and sensitive aptasensor for colorimetric detection of adenosine triphosphate based on unmodified gold nanoparticles. Talanta 168: 279-285, 2018

Chitosan-functionalized gold nanoparticles for colorimetric detection of mercury ions based on chelation-induced aggregation. Microchimica Acta 182(3-4): 611-616, 2015

A novel colorimetric aptasensor for ultrasensitive detection of cocaine based on the formation of three-way junction pockets on the surfaces of gold nanoparticles. Analytica Chimica Acta 1020: 110-115, 2018

Colorimetric assay for protein detection based on "nano-pumpkin" induced aggregation of peptide-decorated gold nanoparticles. Biosensors and Bioelectronics 71: 348-352, 2016

Detection of Malachite Green using a colorimetric aptasensor based on the inhibition of the peroxidase-like activity of gold nanoparticles by cetyltrimethylammonium ions. Mikrochimica Acta 186(5): 322, 2019

Colorimetric detection of L-histidine based on the target-triggered self-cleavage of swing-structured DNA duplex-induced aggregation of gold nanoparticles. Mikrochimica Acta 185(10): 452, 2018