+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by 13 C metabolic flux analysis



Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by 13 C metabolic flux analysis



Metabolic Engineering 39: 9-18



Glucose and xylose are the two most abundant sugars derived from the breakdown of lignocellulosic biomass. While aerobic glucose metabolism is relatively well understood in E. coli, until now there have been only a handful of studies focused on anaerobic glucose metabolism and no 13C-flux studies on xylose metabolism. In the absence of experimentally validated flux maps, constraint-based approaches such as MOMA and RELATCH cannot be used to guide new metabolic engineering designs. In this work, we have addressed this critical gap in current understanding by performing comprehensive characterizations of glucose and xylose metabolism under aerobic and anaerobic conditions, using recent state-of-the-art techniques in 13C metabolic flux analysis (13C-MFA). Specifically, we quantified precise metabolic fluxes for each condition by performing parallel labeling experiments and analyzing the data through integrated 13C-MFA using the optimal tracers [1,2-13C]glucose, [1,6-13C]glucose, [1,2-13C]xylose and [5-13C]xylose. We also quantified changes in biomass composition and confirmed turnover of macromolecules by applying [U-13C]glucose and [U-13C]xylose tracers. We demonstrated that under anaerobic growth conditions there is significant turnover of lipids and that a significant portion of CO2 originates from biomass turnover. Using knockout strains, we also demonstrated that β-oxidation is critical for anaerobic growth on xylose. Quantitative analysis of co-factor balances (NADH/FADH2, NADPH, and ATP) for different growth conditions provided new insights regarding the interplay of energy and redox metabolism and the impact on E. coli cell physiology.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057481619

Download citation: RISBibTeXText

PMID: 27840237

DOI: 10.1016/j.ymben.2016.11.003


Related references

Finding elementary flux modes in metabolic networks based on flux balance analysis and flux coupling analysis: application to the analysis of Escherichia coli metabolism. Biotechnology Letters 35(12): 2039-2044, 2014

Diversion of the metabolic flux from pyruvate dehydrogenase to pyruvate oxidase decreases oxidative stress during glucose metabolism in nongrowing Escherichia coli cells incubated under aerobic, phosphate starvation conditions. Journal of Bacteriology 186(21): 7364-7368, 2004

Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and (13)C metabolic flux analysis. Biotechnology for Biofuels 9(1): 175, 2016

Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13 C metabolic flux analysis. AichE Journal 59(9): 3195-3202, 2013

Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method. Journal of Biotechnology 101(2): 101-117, 6 March, 2003

Quantitative metabolomics and metabolic flux analysis reveal impact of altered trehalose metabolism on metabolic phenotypes of Penicillium chrysogenum in aerobic glucose-limited chemostats. Biochemical Engineering Journal 146: 41-51, 2019

Enhanced production of 3-hydroxypropionic acid from glucose and xylose by alleviation of metabolic congestion due to glycerol flux in engineered Escherichia coli. Bioresource Technology 285: 121320, 2019

Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. I. Growth-rate-dependent metabolic efficiency at steady state. Microbiology 151(Pt 3): 693-706, 2005

The aerobic and anaerobic effect of glucose concentrations on escherichia coli metabolism. Proceedings of the Australian Biochemical Society 6: 30, 1973

Global transcription and metabolic flux analysis of Escherichia coli in glucose-limited fed-batch cultivations. Applied and Environmental Microbiology 74(22): 7002-7015, 2008

Adaptive evolution of Escherichia coli inactivated in the phosphotransferase system operon improves co-utilization of xylose and glucose under anaerobic conditions. Applied Biochemistry and Biotechnology 163(4): 485-496, 2011

Effect of glucose analog supplementation on metabolic flux distribution in anaerobic chemostat cultures of Escherichia coli. Metabolic Engineering 2(2): 149-154, 2000

Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses. Biotechnology and Bioengineering 104(6): 1153-1161, 2010

Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism. Journal of Bacteriology 181(21): 6679-6688, 1999

Contribution to the study of tryptophan metabolism by Escherichia coli. I. Using media with glucose and without glucose and in aerobic conditions. Microbiologia Espanola 20(1): 53-61, 1967