Section 58
Chapter 57,529

Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR

Edouard, S.; Prudent, E.; Gautret, P.; Memish, Z.A.; Raoult, D.

Journal of Clinical Microbiology 53(3): 1002-1004


ISSN/ISBN: 1098-660X
PMID: 25552360
DOI: 10.1128/jcm.03609-14
Accession: 057528008

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We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.

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