+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of KRAS,NRAS and BRAF gene mutations in colorectal carcinoma



Detection of KRAS,NRAS and BRAF gene mutations in colorectal carcinoma



Zhonghua Bing Li Xue Za Zhi 44(4): 254-257



To investigate mutations frequencies of KRAS,NRAS and BRAF genes in colorectal carcinoma. Tissue specimens from 200 colorectal cancer patients at diagnosis were collected and subject to KRAS,NRAS and BRAF mutation analyses by PCR-based direct DNA sequencing targeting exons 2, 3 and 4 of KRAS gene, exons 2, 3 and 4 of NRAS gene and exon 15 of BRAF gene. Activating mutations were detected in KRAS (44%, 88/200), NRAS (2%, 4/200) and BRAF (5%, 10/200) in this study cohort.Among KRAS mutations, 64.8% (57/88) occurred in codon 12 and 12.5% (11/88) occurred in codon 13. KRAS gene mutation in exon 3 mainly involved codons 59 and 61. KRAS gene mutation in exon 4 mainly involved codons 117 and 146. Mutations at exon 2 of KRAS gene have the highest frequency in colorectal carcinoma. Expanding the detection sites of KRAS gene combined with NRAS and BRAF genes may help to identify patients who will most likely benefit from targeted therapies.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 057586453

Download citation: RISBibTeXText

PMID: 25975908


Related references

Detection of KRAS, BRAF, PIK3CA and EGFR gene mutations in colorectal carcinoma. Zhonghua Bing Li Xue Za Zhi 41(9): 590-594, 2013

Correlation Research between Syndrome Elements of Chinese Medicine and KRAS, BRAF, NRAS Gene Mutations in Advanced Colorectal Cancer Patients. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi 36(12): 1445-1448, 2016

KRAS, NRAS and BRAF mutations in colorectal cancer and melanoma. Medical Oncology 34(2): 26-26, 2017

Frequency of KRAS, BRAF, and NRAS mutations in colorectal cancer. Genes, Chromosomes & Cancer 50(5): 307-312, 2011

Molecular spectrum of KRAS, NRAS, BRAF, PIK3CA, TP53, and APC somatic gene mutations in Arab patients with colorectal cancer: determination of frequency and distribution pattern. Journal of Gastrointestinal Oncology 7(6): 882-902, 2017

Can CT-based radiomics signature predict KRAS/NRAS/BRAF mutations in colorectal cancer?. European Radiology: -, 2018

Prognostic role of KRAS, NRAS, BRAF and PIK3CA mutations in advanced colorectal cancer. Future Oncology 11(4): 629-640, 2015

Mutations of KRAS/NRAS/BRAF predict cetuximab resistance in metastatic colorectal cancer patients. Oncotarget 7(16): 22257-22270, 2017

KRAS, NRAS and BRAF mutations in Greek and Romanian patients with colorectal cancer: a cohort study. Bmj Open 4(5): E004652-E004652, 2014

Analysis of KRAS, NRAS, BRAF, and PIK3CA mutations could predict metastases in colorectal cancer: A preliminary study. Advances in Clinical and Experimental Medicine, 2018

Detection of Rare Mutations by Routine Analysis of KRAS, NRAS, and BRAF Oncogenes. Bulletin of Experimental Biology and Medicine 162(3): 375-378, 2017

Prognostic impact of KRAS, NRAS, BRAF, and PIK3CA mutations in primary colorectal carcinomas: a population-based study. Journal of Translational Medicine 14(1): 292-292, 2017

Elucidating the prognostic significance of KRAS, NRAS, BRAF and PIK3CA mutations in Chinese patients with metastatic colorectal cancer. Asia-Pacific Journal of Clinical Oncology 11(2): 160-169, 2016

Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations. Analytical Biochemistry 491: 75-83, 2016

Combined mutational analysis of KRAS, NRAS and BRAF genes in Indian patients with colorectal carcinoma. International Journal of Biological Markers 27(1): 27-33, 2012