+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes



Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes



Journal of Applied Oral Science 24(2): 142-147



Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. Presumptive β-lactamase-producing isolates were evaluated for definite β-lactamase production using the nitrocefin slide method and identified using the API Rapid 32A system. Antimicrobial susceptibility was performed using disc diffusion and microbroth dilution tests as described by CLSI Methods. Isolates were screened for the presence of the β-lactamase-TEM (BlaTEM) and β-lactamase-cfxA genes using Polymerase Chain Reaction (PCR). Amplified PCR products were sequenced and the cfxA gene was characterized using Genbank databases. Results Seventy five percent of patients carried two species of β-lactamase-producing anaerobic bacteria that comprised 9.4% of the total number of cultivable bacteria. Fifty one percent of β-lactamase-producing strains mainly Prevotella, Porphyromonas, and Bacteroides carried the cfxA gene, whereas none of them carried blaTEM. Further characterization of the cfxA gene showed that 76.7% of these strains carried the cfxA2 gene, 14% carried cfxA3, and 9.3% carried cfxA6. The cfxA6 gene was present in three Prevotella spp. and in one Porphyromonas spp. Strains containing cfxA genes (56%) were resistant to the β-lactam antibiotics. Conclusion This study indicates that there is a high prevalence of the cfxA gene in β-lactamase-producing anaerobic oral bacteria, which may lead to drug resistance and treatment failure.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 057587386

Download citation: RISBibTeXText

PMID: 27119762

DOI: 10.1590/1678-775720150469


Related references

Detection of cfxA and cfxA2, the beta-lactamase genes of Prevotella spp., in clinical samples from dentoalveolar infection by real-time PCR. Journal of Clinical Microbiology 44(1): 172-176, 2006

Comparative activity of beta-lactamase inhibitors YTR 830, clavulanate, and sulbactam combined with beta-lactams against beta-lactamase-producing anaerobes. Antimicrobial Agents and ChemoTherapy 30(5): 789-791, 1986

First case of bacteremia due to chromosome-encoded CfxA3-beta-lactamase-producing Capnocytophaga sputigena in a pediatric patient with acute erythroblastic leukemia. European Journal of Medical Research 13(3): 133-135, 2008

Detection of bla(CTX-M) beta-lactamase genes in extended-spectrum beta-lactamase producing gram-negative bacteria. Mikrobiyoloji Bulteni 44(2): 187-196, 2011

In vitro activity of ceftazidime-NXL104 against 396 strains of beta-lactamase-producing anaerobes. Antimicrobial Agents and ChemoTherapy 55(7): 3616-3620, 2011

Investigation of beta-lactamase genes and clonal relationship among the extended-spectrum beta-lactamase producing nosocomial Escherichia coli isolates. Mikrobiyoloji Bulteni 49(1): 15-25, 2015

Beta lactamase stability and antibacterial activities of oral cephalosporins compared with amoxycillin clavulanic acid against beta lactamase producing moraxella catarrhalis. Program & Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 31: 119, 1991

Use of beta-lactamase-producing anaerobes to prevent ceftriaxone from degrading intestinal resistance to colonization. Journal of Infectious Diseases 160(2): 274-280, 1989

The first detection of CTX-M-14 extended-spectrum beta-lactamase among diverse beta-lactamase-producing Proteus mirabilis clinical isolates. Diagnostic Microbiology and Infectious Disease 54(3): 237-239, 2006

First detection of VIM-1 type metallo-beta-lactamase in a multidrug-resistant Klebsiella pneumoniae clinical isolate from Turkey also producing the CTX-M-15 extended-spectrum beta-lactamase. Journal of ChemoTherapy 19(4): 467-468, 2007

Tonsil surface and core cultures in recurrent tonsillitis: Prevalence of anaerobes and beta-lactamase producing organisms. European Journal of Clinical Microbiology & Infectious Diseases 13(7): 542-548, 1994

Real-time PCR for fast detection of plasmid-mediated qnr genes in extended spectrum beta-lactamase producing Enterobacteriaceae. Pathologie-Biologie 58(6): 430-433, 2011

Detection of New Delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in Escherichia coli isolated from mastitic milk samples. Transboundary and Emerging Diseases 60(5): 385-389, 2014