+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of virgin coconut oil adulteration with animal fats using quantitative cholesterol by GC × GC-TOF/MS analysis

Detection of virgin coconut oil adulteration with animal fats using quantitative cholesterol by GC × GC-TOF/MS analysis

Food Chemistry 178: 128-135

A new method based on the cholesterol level was developed to detect the presence of animal fats in virgin coconut oil (VCO). In this study, the sterols in VCO and animal fats was separated using conventional one-dimensional gas chromatography (1D GC) and comprehensive two-dimensional gas chromatography (GC×GC). Compared with 1D GC, the GC×GC system could obtain a complete baseline separation of the sterol trimethylsilyl ethers derived from cholesterol and cholestanol, so that the cholesterol content in pure VCO and false VCO adulterated with animal fats could be accurately determined. Cholesterol, a main sterol found in animal fats, represented less than 5mg/kg of VCO. The study demonstrated that the determination of the cholesterol level in VCO could be used for reliable detection of the presence of lard, chicken fat, mutton tallow, beef tallow, or their mixture in VCO at a level as little as 0.25%.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057588696

Download citation: RISBibTeXText

PMID: 25704693

DOI: 10.1016/j.foodchem.2015.01.035

Related references

Adulteration of Argentinean milk fats with animal fats: Detection by fatty acids analysis and multivariate regression techniques. Food Chemistry 192: 1025-1032, 2016

Analysis of adulteration of virgin coconut oil by palm kernel olein using fourier transform infrared spectroscopy. Journal of food lipids 14(2): 111-121, 2007

Detection of refined animal and vegetable fats in adulteration of pure milkfat. Journal of the American Oil Chemists' Society 71(12): 1381-1384, 1994

Detection of adulteration in ghee with animal body fats and vegetable oils using opacity test. Journal of Dairying, Foods and Home Sciences 17(1): 31-36, 1998

Detection of adulteration of vegetable oils and hydrogenated oil with animal body fats by opacity test. Journal of Food Science and Technology 35(6): 549-550, 1998

Determination of Milk Fat Adulteration with Vegetable Oils and Animal Fats by Gas Chromatographic Analysis. Journal of Food Science 80(9): C1945, 2015

Blending and adulteration of fats. Possibility of detection by stearin analysis. Forschungs Report, Ernahrung Landwirtschaft Forsten ( 5): 10-12, 1990

Detection of Adulteration of Exta-Virgin Olive Oil by Chemometric Analysis of Mid-Infrared Spectral Data. 2009

Monitoring the adulteration of virgin coconut oil by selected vegetable oils using differential scanning calorimetry. Journal of Food Lipids 16(1): 50-61, 2009

Use of the SAW sensor electronic nose for detecting the adulteration of virgin coconut oil with RBD palm kernel olein. Journal Of The American Oil Chemists' Society: 3, 263-270, 2010

Adulteration of milk products review of the analytical criteria for the detection of vegetable and animal fats in milk fat. Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene 82(6): 615-662, 1991

Sterol fractions in hazelnut and virgin olive oils and 4,4'-dimethylsterols as possible markers for detection of adulteration of virgin olive oil. Journal of the American Oil Chemists' Society 82(10): 717-725, 2005

The effect of thermal treatment on the enhancement of detection of adulteration in extra virgin olive oils by synchronous fluorescence spectroscopy and chemometric analysis. Spectrochimica Acta. Part A Molecular and Biomolecular Spectroscopy 161: 83-87, 2016

MALDI-TOF mass spectrometry detection of extra-virgin olive oil adulteration with hazelnut oil by analysis of phospholipids using an ionic liquid as matrix and extraction solvent. Food Chemistry 134(2): 1192-1198, 2012

Quantitative analysis of virgin coconut oil in cream cosmetics preparations using fourier transform infrared (FTIR) spectroscopy. Pakistan Journal of Pharmaceutical Sciences 22(4): 415-420, 2009