+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of PCR and TaqMan PCR Assays to Detect Pseudomonas coronafaciens, a Causal Agent of Halo Blight of Oats

Development of PCR and TaqMan PCR Assays to Detect Pseudomonas coronafaciens, a Causal Agent of Halo Blight of Oats

Plant Pathology Journal 31(1): 25-32

Pseudomonas coronafaciens causes halo blight on oats and is a plant quarantine bacterium in many countries, including the Republic of Korea. Using of the certificated seed is important for control of the disease. Since effective detection method of P. coronafaciens is not available yet, PCR and TaqMan PCR assays for specific detection of P. coronafaciens were developed in this study. PCR primers were designed from the draft genome sequence of P. coronafaciens LMG 5060 which was obtained by the next-generation sequencing in this study. The PCR primer set Pc-12-F/Pc-12-R specifically amplified 498 bp from the 13 strains of P. coronafaciens isolated in the seven different countries (Canada, Japan, United Kingdom, Zimbabwe, Kenya, Germany, and New Zealand) and the nested primer set Pc-12-ne-F/Pc-12-ne-R specifically amplified 298 bp from those strains. The target-size PCR product was not amplified from the non-target bacteria with the PCR and nested primer sets. TaqMan PCR with Pc-12-ne-F/Pc-12-ne-R and a TaqMan probe, Pc-taqman, which were designed inside of the nested PCR amplicon, generated Ct values which in a dose-dependent manner to the amount of the target DNA and the Ct values of all the P. coronafaciens strains were above the threshold Ct value for positive detection. The TaqMan PCR generated positive Ct values from the seed extracts of the artificially inoculated oat seeds above 10 cfu/ml inoculation level. PCR and TaqMan PCR assays developed in this study will be useful tools to detect and identify the plant quarantine pathogen, P. coronafaciens.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057599010

Download citation: RISBibTeXText

PMID: 25774107

DOI: 10.5423/ppj.oa.09.2014.0096

Related references

Pseudomonas syringae pv. coronafaciens (Elliot) Young, Dye & Wilkie, causal agent of bacterial halo blight of oats, indentified in Region IX, Chile. Agricultura Tecnica 45(1): 73-74, 1985

The occurrence of halo blight of oats avena sativa caused by pseudomonas syringae pathovar coronafaciens. Fitopatologia Brasileira 11(3): 737-741, 1986

Epiphytic phase of existence of Pseudomonas syringae pv. coronafaciens, the oats halo bacteriosis agent. Mikrobiologichnii Zhurnal 61(6): 9-14, 1999

Free amino acid and amide content of tobacco and oats infected by wildfire and halo blight bacteria , Pseudomonas coronafaciens. Phytopathology 53(8): 885, 1963

Halo blight of oats produced by the bacterium Ps. coronafaciens (Elliott) Stevens. Probl. Agric, Bucuresti, 17: 3, 77-82, 1965

On the first occurrence of bacterial leaf-withering ('halo blight') (P. coronafaciens) on Oats in Germany. Z. PflKrankh 66: 11-12, 676-680, 1959

Studies on the causal agent of coffee halo blight (Pseudomonas garcae Amarel et al.). Arquivos da Universidade Federal Rural do Rio de Janeiro 3(2): 15-18, 1973

Morphological variation of colonies of Pseudomonas syringae pv. coronafaciens causing halo burn of oats. Sel' skokhozyaistvennaya Biologiya ( 1): 103-106, 1989

Bacterial halo blight, a new disease of rye caused by Pseudomonas syringae pv. coronafaciens. Japanese Journal of Phytopathology 69(2): 136-139, 2003

Halo blight (Pseudomonas syringae pv. coronafaciens) on grasses in interior Alaska. Plant Disease 70(8): 801, 1986

Potential of bacteriocins for control of Pseudomonas phaseolicola, causal agent of halo blight of beans. Annual report: 17 92, 1974

The causal agent of halo blight in bean, Pseudomonas syringae pv. phaseolicola, attaches to stomata via its pili. Microbial Pathogenesis 1(2): 139-148, 1986

Bacteriophages in the biological control of Pseudomonas syringae pv. phaseolicola, causal agent of halo blight in bean. Ecosistemas y Recursos Agropecuarios 5(14): 191-202, 2018

Effect of irradiance upon the population of Pseudomonas coronafaciens in leaves and symptom expression of halo blight of rye. Canadian Journal of Microbiology 25(2): 163-166, 1979

The causal agent of halo blight in bean, Pseudomonas syringae pathovar phaseolicola, attaches to stomata via its pili. Microb Path 1(2): 139-148, 1986