+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of hair cells in inner ear is associated with expression and promoter methylation of Notch-1 in postnatal mice

Development of hair cells in inner ear is associated with expression and promoter methylation of Notch-1 in postnatal mice

International Journal of Clinical and Experimental Medicine 8(9): 15542-8

The present study was designed to investigate the correlation among the number of hair cells in inner ear, Notch-1 gene expression levels and its methylation status of the promoter region in the postnatal mice. The hair cells in inner ear were collected from postnatal mice at day 0, 4, 8 and 16 and counted by immunofluorescence. Notch-1 mRNA expression were measured by real-time quantitative polymerize chain reaction (PCR). Methylation levels of CpG islands in Notch-1 promoters were analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The results showed that the number of hair cells in the inner ear increased gradually after birth, which were positively correlated to Notch-1 mRNA expression. However, analysis on methylation of CpG sites in Notch-1 promoter showed that the methylation rates increased gradually after births, which were correlated with the decreased expression of Notch-1. Drug lesion induced the loss of hair cells, and stimulated the expression of Notch-1 mRNA expression, but didn't influence the methylation rates of Notch-1 promoter. We concluded that the Notch-1 mRNA expression level in inner ear tissues is correlated with the development of hair cells. CpG islands in Notch-1 promoter region manifest hypermethylation status when hair cells in inner ear are mature.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 057603569

Download citation: RISBibTeXText

PMID: 26629046

Related references

Exploring the potential relationship between Notch pathway genes expression and their promoter methylation in mice hippocampal neurogenesis. Brain Research Bulletin 113: 8-16, 2016

Expression of notch and jagged correlates with development of hair cells in the organ of Corti. Society for Neuroscience Abstracts 23(1-2): 1968, 1997

Sodium current expression during postnatal development of rat outer hair cells. Pflugers Archiv 434(6): 772-778, 1997

In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation. Developmental Dynamics 241(4): 684-696, 2013

Expression of the p63 and Notch signaling systems in rat testes during postnatal development: comparison with their expression levels in the epididymis and vas deferens. Journal of Andrology 25(5): 692-698, 2004

Promoter histone H3 lysine 9 di-methylation is associated with DNA methylation and aberrant expression of p16 in gastric cancer cells. Oncology Reports 22(5): 1221-1227, 2009

Differential Promoter Methylation of Macrophage Genes Is Associated With Impaired Vascular Growth in Ischemic Muscles of Hyperlipidemic and Type 2 Diabetic Mice: Genome-Wide Promoter Methylation Study. Circulation Research 117(3): 289-299, 2015

Auditory hair cell-specific deletion of p27Kip1 in postnatal mice promotes cell-autonomous generation of new hair cells and normal hearing. Journal of Neuroscience 34(47): 15751-15763, 2015

Expression of a potassium current in inner hair cells during development of hearing in mice. Nature 394(6690): 281-284, 1998

Immunolocalization of β-catenin and Lef-1 during postnatal hair follicle development in mice. Acta Histochemica 114(8): 773-778, 2014

Requirement of DHCR24 for postnatal development of epidermis and hair follicles in mice. American Journal of Dermatopathology 31(5): 446-452, 2009

CD34 expression by hair follicle stem cells is required for skin tumor development in mice. Cancer Research 67(9): 4173-4181, 2007

Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. Glia 56(11): 1224-1237, 2008

Induction of the hair growth phase in postnatal mice by localized transient expression of Sonic hedgehog. Journal of Clinical Investigation 104(7): 855-864, 1999

Possible involvement of DNA methylation in NKCC1 gene expression during postnatal development and in response to ischemia. Journal of Neurochemistry 114(2): 520-529, 2010