+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus



Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus



Parasites and Vectors 9(1): 524



In eukaryotic organisms, Piwi-interacting RNAs (piRNAs) control the activities of mobile genetic elements and ensure genome maintenance. Recent evidence indicates that piRNAs are involved in multiple biological pathways, including transcriptional regulation of protein-coding genes, sex determination and even interactions between host and pathogens. Aedes albopictus is a major invasive species that transmits a number of viral diseases in humans. Ae. albopictus has the largest genome and the highest abundance of repetitive sequences when compared with members that belong to Culicidae with a published genome. Analysis of piRNA profiles will provide a developmental and evolutionary perspective on piRNAs in Ae. albopictus. piRNAs were identified and characterized during the development of Ae. albopictus, and piRNA expression patterns in adult males and females as well as sugar-fed females and blood-fed females were compared. Our results reveal that, despite the large genome size of Ae. albopictus, the piRNA pool of Ae. albopictus (1.2 × 107) is smaller than those of Aedes aegypti (1.7 × 107) and Drosophila melanogaster (1.6 × 107). In Ae. albopictus, piRNAs displayed the highest abundance at the embryo stage and the lowest abundance at the pupal stage. Approximately 50 % of the piRNAs mapped to intergenic regions with no known functions. Approximately 30 % of the piRNAs mapped to repetitive elements, and 77.69 % of these repeat-derived piRNAs mapped to Class I TEs; 45.42 % of the observed piRNA reads originated from piRNA clusters, and most of the top 10 highest expressed piRNA clusters and 100 highest expressed piRNAs from each stage displayed biased expression patterns across the developmental stages. All anti-sense-derived piRNAs displayed a preference for uridine at the 5' end; however, the sense-derived piRNAs showed adenine bias at the tenth nucleotide position and a typical ping-pong signature, suggesting that the biogenesis of piRNAs was conserved throughout development. Our results also show that 962 piRNAs displayed sex-biased expression, and 522 piRNAs showed higher expression in the blood-fed females than in the sugar-fed females. Our results suggest that piRNAs, aside from silencing transposable elements in Ae. albopictus, may have a role in other biological pathways.

(PDF emailed within 0-6 h: $19.90)

Accession: 057606151

Download citation: RISBibTeXText

PMID: 27686069

DOI: 10.1186/s13071-016-1815-8


Related references

miRNA genes of an invasive vector mosquito, Aedes albopictus. Plos One 8(7): E67638, 2014

Molecular and functional characterization of odorant-binding protein genes in an invasive vector mosquito, Aedes albopictus. Plos One 8(7): E68836, 2014

Effects of Cohabitation on the Population Performance and Survivorship of the Invasive Mosquito Aedes albopictus and the Resident Mosquito Aedes notoscriptus (Diptera: Culicidae) in Australia. Journal of Medical Entomology 52(3): 375-385, 2015

Seasonal photoperiods alter developmental time and mass of an invasive mosquito, Aedes albopictus (Diptera: Culicidae), across its north-south range in the United States. Journal of Medical Entomology 49(4): 825-832, 2012

The developmental transcriptome of the mosquito Aedes aegypti, an invasive species and major arbovirus vector. G3 3(9): 1493-1509, 2014

Aedes albopictus and Aedes japonicus - two invasive mosquito species with different temperature niches in Europe. Parasites and Vectors 9(1): 573, 2017

Testing predictions of displacement of native Aedes by the invasive Asian Tiger Mosquito Aedes albopictus in Florida, USA. Biological Invasions 3(2): 151-166, 2001( ), 2002

Evaluating the Vector Control Potential of the In2Care® Mosquito Trap Against Aedes aegypti and Aedes albopictus Under Semifield Conditions in Manatee County, Florida. Journal of the American Mosquito Control Association 33(3): 193-199, 2018

Possible jungle dengue recent studies and hypotheses distribution human primary host mosquito vector virus aedes aegypti monkey aedes albopictus. Japanese Journal of Medical Science & Biology 20(SUPP): 69-74, 1967

Mosquito Surveillance and the First Record of the Invasive Mosquito Species Aedes ( Stegomyia ) albopictus (Skuse) (Diptera: Culicidae) in Southern Iran. Iranian Journal of Public Health 45(8): 1064-1073, 2016

Invasive leaf resources alleviate density dependence in the invasive mosquito, Aedes albopictus. Biological Invasions 12(7): 2319-2328, 2010

Aedes albopictus, an exotic mosquito vector in the United States. Annals of Internal Medicine 105(3): 449-451, 1986

Field prevalence of Wolbachia in the mosquito vector Aedes albopictus. American Journal of Tropical Medicine and Hygiene 66(1): 108-111, 2002

Effect of Polygonum hydropiper L. against dengue vector mosquito Aedes albopictus L. Parasitology Research 113(9): 3143-3150, 2014

How does the dengue vector mosquito Aedes albopictus respond to global warming?. Parasites and Vectors 10(1): 140, 2017