Home
  >  
Section 58
  >  
Chapter 57,620

Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques

Dorado, J.; Gálvez, M.J.; Demyda-Peyrás, S.; Ortiz, I.; Morrell, J.M.; Crespo, F.; Gósalvez, J.; Hidalgo, M.

Reproduction Fertility and Development 2015

2015

ISSN/ISBN: 1031-3613
PMID: 25879166
DOI: 10.1071/rd15071
Accession: 057619200
Download citation:  
Text
  |  
BibTeX
  |  
RIS
This study compared the efficacy of simple sperm washing (SW), single-layer centrifugation (SLC) and modified swim-up (SU) techniques in the preparation of dog spermatozoa for cooling. Eighteen ejaculates, collected from three dogs (six per dog), were pooled (three ejaculates per pool) and divided into three aliquots: (1) one aliquot was washed and cooled at 5°C for 72h, considered as control (SW-control), (2) the second aliquot was selected by SLC through Androcoll-C and subsequently cooled in the same way as the SW-control samples (SLC-AC) and (3) the last aliquot was selected by a modified SU method with Androcoll-C and cooled as mentioned above (SU-AC). Assessment of sperm motility, sperm morphology, sperm membrane integrity and acrosome integrity were performed on aliquots of fresh semen and chilled-rewarmed samples. Sperm membrane integrity and progressive motility were significantly (PPP>0.05). The recovery rates were not significantly (P>0.05) different between SW-control, SLC-AC and SU-AC samples. Our results confirm that SU-AC may be a successful method for the preparation of dog spermatozoa for cooling.

PDF emailed within 0-6 h: $19.90




Related References

Malo, C.; Crichton, E.G.; Morrell, J.M.; Pukazhenthi, B.S.; Skidmore, J.A. 2017: Single layer centrifugation of fresh dromedary camel semen improves sperm quality and in vitro fertilization capacity compared with simple sperm washing Reproduction in Domestic Animals 52(6): 1097-1103
Fácio, C.ás.L.; Previato, L.íg.F.; Machado-Paula, L.A.; Matheus, P.C.; Araújo, E. 2016: Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation Jbra Assisted Reproduction 20(4): 206-211
Dorado, J.; Galvez, M. J.; Murabito, M. R.; Quintero, A.; Hidalgo, M. 2009: Effect of Egg Yolk and Centrifugation on Sperm Motility of Canine Chilled Semen Reproduction in Domestic Animals 44: 101
Wong, P.C.; Balmaceda, J.P.; Blanco, J.D.; Gibbs, R.S.; Asch, R.H. 1986: Sperm washing and swim-up technique using antibiotics removes microbes from human semen Fertility and Sterility 45(1): 97-100
Ricci, G.; Perticarari, S.; Boscolo, R.; Montico, M.; Guaschino, S.; Presani, G. 2009: Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique Fertility and Sterility 91(2): 632-638
Morrell, J.M.; Rodriguez-Martinez, H.; Johannisson, A. 2010: Single layer centrifugation of stallion spermatozoa improves sperm quality compared with sperm washing Reproductive Biomedicine Online 21(3): 429-436
Lopes, G.; Simões, A.; Ferreira, P.; Martins-Bessa, A.; Rocha, A. 2009: Differences in preservation of canine chilled semen using different transport containers Animal Reproduction Science 112(1-2): 158-163
Urbano, M.; Dorado, J.; Ortiz, I.; Morrell, J.M.; Demyda-Peyrás, S.; Gálvez, M.J.; Alcaraz, L.; Ramírez, L.; Hidalgo, M. 2013: Effect of cryopreservation and single layer centrifugation on canine sperm DNA fragmentation assessed by the sperm chromatin dispersion test Animal Reproduction Science 143(1-4): 118-125
Malo, C.; Crichton, E. G.; Morrell, J. M.; Pukazhenthi, B. S.; Skidmore, J. A. 2018: Use of Single Layer Centrifugation to select sperm in dromedary camel semen Reproduction in Domestic Animals 53: 81
Chen, S.U.; Ho, H.N.; Chen, H.F.; Chao, K.H.; Lin, H.R.; Huang, S.C.; Lee, T.Y.; Yang, Y.S. 1995: Comparison between a two-layer discontinuous Percoll gradient and swim-up for sperm preparation on normal and abnormal semen samples Journal of Assisted Reproduction and Genetics 12(10): 698-703
Soffer, Y.; Ron, E.R.; Hagai, N.; Weinraub, Z.; Caspi, E. 1986: Sperm variables following sperm washing and swim up in patients with low semen quality Journal of In Vitro Fertilization and Embryo Transfer 3(2): 72-73
Morrell, J.; Garcia, B. M.; Pena, F.; Johannisson, A.; Meurling, S. 2011: Single layer centrifugation of cooled semen with androcoll-e improves sperm quality Reproduction in Domestic Animals 46: 132-132
Neulen, J.; Weitzell, R.; Breckwoldt, M. 1987: The effect of centrifugation on sperm motility after washing spermatozoa by a swim up technique Human Reproduction 2(Suppl 1): 111
Dorado, J.; Gálvez, M.J.; Morrell, J.M.; Alcaráz, L.; Hidalgo, M. 2013: Use of single-layer centrifugation with Androcoll-C to enhance sperm quality in frozen-thawed dog semen Theriogenology 80(8): 955-962
Repalle, D.; Chittawar, P.B.; Bhandari, S.; Joshi, G.; Paranjape, M.; Joshi, C. 2013: Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? Journal of Human Reproductive Sciences 6(1): 23-26