+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Differential regulation of oxytocin receptor in various adipose tissue depots and skeletal muscle types in obese Zucker rats

Differential regulation of oxytocin receptor in various adipose tissue depots and skeletal muscle types in obese Zucker rats

Hormone and Metabolic Research 47(8): 600-604

Multifunctional peptide oxytocin currently undergoes intensive research due to its proposed anti-obesity properties. Until now, little is known about regulation of oxytocin receptor in metabolically active tissues in obesity. The aim of the present study was to measure expression of oxytocin receptor upon obese phenotype with respect to the variety among adipose tissue and skeletal muscles with distinct anatomical localisation. Total homogenates were prepared from epididymal, retroperitoneal and inguinal adipose tissues as well as quadriceps and soleus muscle from lean and obese Zucker rats. Oxytocin receptor protein was determined by immunoblot. Interestingly, elevated oxytocin receptor was observed in epididymal adipose tissue of obese rats in contrast to its downregulation in subcutaneous and no change in retroperitoneal fat. In lean animals, oxytocin receptor protein was expressed at similar levels in all adipose depots. This uniformity was not observed in the case of skeletal muscle in which fibre type composition seems to be determinant of oxytocin receptor expression. Quadriceps muscle with the predominance of glycolytic fibres exhibits higher oxytocin receptor expression than almost exclusively oxidative soleus muscle. Oxytocin receptor protein levels were decreased in both skeletal muscles analysed upon obese phenotype. The present work demonstrates that even under identical endocrine circumstances, oxytocin receptor is differentially regulated in adipose tissue of obese rats depending on fat depot localisation. These results also imply which tissues may be preferentially targeted by oxytocin treatment in metabolic disease.

Accession: 057623737

Download citation: RISBibTeXText

PMID: 25565097

DOI: 10.1055/s-0034-1395677

Related references

Tissue specific regulation of glucose transporters in adipose cells and muscle of obese zucker rats. Sakamoto, N , A Angel And N Hotta (Ed ) International Congress Series, No 963 New Directions in Research And Clinical Works For Obesity And Diabetes Mellitus; Satellite Symposium Of The 6th International Congress Of Obesity: Obesity And Diabetes Mellitus, Nagoya, Japan, October 18-19, 1990 Xviii+411p Elsevier Science Publishers B V : Amsterdam, Netherlands; (Dist in The Usa And Canada By Elsevier Science Publishing Co , Inc : New York, New York, Usa) Illus 225-227, 1991

The influence of starvation and refeeding on the lipoprotein lipase activity of skeletal muscle and adipose tissue of lean and obese Zucker rats. Journal of Nutrition 113(6): 1150-1156, 1983

Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue. Journal of Endocrinology 220(3): 333-343, 2014

Differential polypeptide expression in adipose tissue of lean and obese zucker rats evidence of specifically repressed peptides in 7 day old pre obese rats. Biochemical Journal 284(3): 813-817, 1992

Differential regulation of mouse uncoupling proteins among brown adipose tissue, white adipose tissue, and skeletal muscle in chronic beta 3 adrenergic receptor agonist treatment. Biochemical and Biophysical Research Communications 253(1): 85-91, 1999

Differential adipose tissue inflammatory state in obese nondiabetic Zucker fatty rats compared to obese diabetic zucker diabetic fatty rats. Hormone and Metabolic Research 44(4): 273-278, 2012

Defective sympathetic regulation of brown adipose tissue of zucker obese rats. Proceedings of the Nutrition Society 43(2): 68A, 1984

Regulation of adipose tissue metabolism by insulin sensitizers in obese Zucker rats in vivo. Diabetologia 43(Supplement 1): A24, August, 2000

The effects of treadmill exercise on expression of UCP-2 of brown adipose tissue and TNF-α of soleus muscle in obese Zucker rats. Journal of Exercise Nutrition & Biochemistry 17(4): 199-207, 2015

The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line. Obesity Research & Clinical Practice 5(1): E1-E78, 2011

Regulation of insulin receptor substrate-1 in the liver, skeletal muscle and adipose tissue of rats throughout pregnancy. Gynecological Endocrinology 17(3): 187-197, 2003

Blueberry intake alters skeletal muscle and adipose tissue peroxisome proliferator-activated receptor activity and reduces insulin resistance in obese rats. Journal of Medicinal Food 14(12): 1511-1518, 2012

Morphological and beta adreno receptor binding abnormalities in brown adipose tissue from obese zucker rats. Federation Proceedings 41(3): ABSTRACT 1104, 1982