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Downregulation of Human Adenovirus DNA Polymerase Gene by Modified siRNAs

Downregulation of Human Adenovirus DNA Polymerase Gene by Modified siRNAs

Molekuliarnaia Biologiia 50(1): 188-192

ISSN/ISBN: 0026-8984

PMID: 27028826

Human adenoviruses, in particular D8, D19, and D37, cause ocular infections. Currently, there is no available causally directed treatment, which efficiently counteracts adenoviral infectious diseases. In our previous work, we showed that gene silencing by means of RNA interference is an effective approach for downregulation of human species D adenoviruses replication. In this study, we compared the biological activity of siRNAs and their modified analogs targeting human species D adenoviruses DNA polymerase. We found that one of selectively 2'-O-methyl modified siRNAs mediates stable and long-lasting suppression of the target gene (12 days post transfection). We suppose that this siRNA can be used as a potential therapeutic agent against human species D adenoviruses.

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Accession: 057659971

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