+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy

Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy

Applied Optics 54(17): 5425-5431

Dual-mode super-resolution imaging system with two different super-resolution imaging methods, STED and FED, is presented. Electrical shutters controlled by the host computer are introduced to switch the two imaging modes. Principles of both methods are analyzed theoretically, and enhancements in the lateral resolution and SNR are demonstrated theoretically and experimentally. Results show that both imaging methods offered by the proposed system can break the diffraction barrier. Furthermore, the presented system provides a meaningful way to image fluorescent samples by a corresponding imaging mode according to the specific characteristics of samples analyzed for study. For samples that can endure high-power illumination, it is appropriate to use the STED mode to achieve a better resolution, while for samples that are vulnerable to high intensity, the FED method is a better choice because no high-power beam is needed, and the FED method can provide better resolution than STED when no high-power beam is allowed. The flexible switching of the two super-resolution imaging modes can help researchers to make most of the advantages of each imaging method. It is believed that the presented system has the potential to be widely used in future nanoscale investigations.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057667590

Download citation: RISBibTeXText

PMID: 26192843

DOI: 10.1364/ao.54.005425

Related references

STED microscopy--super-resolution bio-imaging utilizing a stimulated emission depletion. Microscopy 64(4): 227-236, 2015

Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy. Optics Express 22(10): 12398-12409, 2014

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Letters 19(11): 780-782, 1994

Analysis of the complexity of the sperm acrosomal membrane by super-resolution stimulated emission depletion microscopy compared with transmission electron microscopy. Microscopy 64(4): 279-287, 2016

Super-resolution imaging prompts re-thinking of cell biology mechanisms: selected cases using stimulated emission depletion microscopy. Bioessays 34(5): 386-395, 2012

Super-resolution stimulated emission depletion microscopy of director structures in liquid crystals. Optics Letters 43(20): 5158-5161, 2018

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging. Optics Letters 33(2): 113-115, 2008

Effects of coherence and vector properties of the light on the resolution limit in stimulated emission depletion fluorescence microscopy. Journal of the Optical Society of America. A, Optics, Image Science, and Vision 25(6): 1378-1382, 2008

Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes. Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics 64(6 Pt 2): 066613, 2001

Super-resolution imaging of lateral distribution for the blue-light emission of an InGaN single-quantum-well structure utilizing the stimulated emission depletion effect. Optics Express 22(19): 22575-22582, 2015

Unraveling complex nanoscale lipid dynamics in simple model biomembranes: Insights from fluorescence correlation spectroscopy in super-resolution stimulated emission depletion mode. Methods 140-141: 198-211, 2017

Fluorescence emission difference microscopy by super-oscillation excitation. Journal of Microscopy 2019, 2019

A 3D aligning method for stimulated emission depletion microscopy using fluorescence lifetime distribution. Microscopy Research and Technique 77(11): 935-940, 2014

Tuning donut profile for spatial resolution in stimulated emission depletion microscopy. Review of Scientific Instruments 84(4): 043701, 2013

The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles. Optics Express 20(12): 12793-8, 2012