EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Detection and characterization of early plaque formations by Raman probe spectroscopy and optical coherence tomography: an in vivo study on a rabbit model



Detection and characterization of early plaque formations by Raman probe spectroscopy and optical coherence tomography: an in vivo study on a rabbit model



Journal of Biomedical Optics 23(1): 1-6



Intravascular imaging techniques provide detailed specification about plaque appearance and morphology, but cannot deliver information about the biochemical composition of atherosclerotic plaques. As the biochemical composition is related to the plaque type, important aspects such as the risk of a plaque rupture and treatment are still difficult to assess. Currently, various spectroscopic techniques are tested for potential applications for the chemical analysis of plaque depositions. Here, we employ Raman spectroscopy in combination with optical coherence tomography (OCT) for the characterization of plaques on rabbits in vivo. Experiments were carried out on New Zealand white rabbits treated with a fat- and cholesterol-enriched diet, using a Raman probe setup with a 785-nm multimode laser as an excitation source. Subsequently, OCT images were acquired with a swept source at 1305±55  nm at 22.6 mW. Raman spectra were recorded from normal regions and regions with early plaque formations. The probe positioning was monitored by x-ray angiography. The spectral information identified plaque depositions consisting of lipids, with triglycerides as the major component. Afterward, OCT images of the spectroscopically investigated areas were obtained. The spectral information correlates well with the observed intravascular morphology and is in good agreement with histology. Raman spectroscopy can provide detailed biochemical specification of atherosclerotic plaques.

(PDF emailed within 0-6 h: $19.90)

Accession: 057790974

Download citation: RISBibTeXText

PMID: 29318812

DOI: 10.1117/1.JBO.23.1.015004



Related references

Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy. Journal of Biomedical Optics 10(3): 031118-031118, 2005

In vivo characterization of atherosclerotic plaque depositions by Raman-probe spectroscopy and in vitro coherent anti-stokes Raman scattering microscopic imaging on a rabbit model. Analytical Chemistry 84(18): 7845-7851, 2013

In vivo characterization of the structure and components of lesional psoriatic skin from the observation with Raman spectroscopy and optical coherence tomography: a pilot study. Journal of Dermatological Science 57(1): 66-69, 2010

Macrophage content detection in an experimental rabbit model of atherosclerotic plaque by optical coherence tomography. Zhonghua Xin Xue Guan Bing Za Zhi 43(2): 146-152, 2015

Classification of burn injury using Raman spectroscopy and optical coherence tomography: An ex-vivo study on porcine skin. Burns, 2018

Detection of Plaque Neovascularization by Optical Coherence Tomography: Ex Vivo Feasibility Study and In Vivo Observation in Patients With Angina Pectoris. Journal of Invasive Cardiology 28(1): 17-22, 2015

Optical coherence tomography and Raman spectroscopy of the ex-vivo retina. Journal of Biophotonics 2(6-7): 398-406, 2009

Diagnostic accuracy of optical coherence tomography and intravascular ultrasound for the detection and characterization of atherosclerotic plaque composition in ex-vivo coronary specimens: a comparison with histology. Coronary Artery Disease 17(5): 425-430, 2006

Non-invasive diagnostic system and its opto-mechanical probe for combining confocal Raman spectroscopy and optical coherence tomography. Journal of Biophotonics 10(11): 1442-1449, 2017

Combined Raman spectroscopy and optical coherence tomography device for tissue characterization. Optics Letters 33(10): 1135-1137, 2008

In vivo optical coherence tomography detection of differences in regional large airway smoke inhalation induced injury in a rabbit model. Journal of Biomedical Optics 13(3): 034001-034001, 2008

Optical coherence tomography for serial in vivo imaging of aortic plaque in the rabbit: a preliminary experience. Open Heart 2(1): E000314-E000314, 2015

In vivo characterization of coronary atherosclerotic plaque by use of optical coherence tomography. Circulation 111(12): 1551-1555, 2005

Optical clearing of melanoma in vivo: characterization by diffuse reflectance spectroscopy and optical coherence tomography. Journal of Biomedical Optics 21(8): 081210-081210, 2016

Development of a hybrid Raman spectroscopy and optical coherence tomography technique for real-time in vivo tissue measurements. Optics Letters 41(13): 3045-3048, 2016