+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers

Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers

Iranian Journal of Public Health 43(7): 947-960

Diarrheal diseases can be caused by viral, bacterial and parasitic infections. This paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in Tunisian children less than five years of age. Overall, 124 diarrhoeal stools were collected from patients suffering from acute diarrhea and 54 stool samples from healthy children. All stools were examined for the presence of enteric pathogens. In diarrheagenic children, 107 pathogenic bacteria were isolated (12 Salmonella spp. (9.7%) and 95 diarrheagenic Escherichia coli strains (76.6%): 29 enteroaggregative E.coli (EAEC) (23.4%), 15 enteroinvasive E.coli (EIEC) (12.1%), 17 enteropathogenic E.coli (EPEC) (13.7%), 26 enterotoxigenic E.coli (ETEC) (21%) and 2 enterohemoragic E.coli (EHEC) (1.6%). However, in the control group, 23 pathogenic E.coli strains were isolated (42.6%): 8 EAEC (14.8%), 12 EIEC (22.2%) and 3 EPEC (5.5%). Among diarrheagenic E.coli (DEC), only ETEC strains were significantly recovered from diarrheagenic children than from healthy controls (P < 0.0003). Group A rotavirus was identified in 33.9% (n=42) of diarrheagenic children and in 11.1% among the control group (n=6). Concerning norovirus, 8.9% (n=11) of the samples collected from diarrheagenic children and 9.2% (n=5) from the control group were positive. The prevalence of rotaviruses and Salmonella spp were also significantly higher in patients with diarrhea than in controls (P = 0.002 and P < 0.019, respectively). Finally, enteropathogenic parasites (Entamoeba coli and cryptosporidium Oocystes) were isolated from 4.8% and 9.2% of diarrheagenic and control children, respectively. These results provide baseline data about the relative importance of different enteropathogens in Tunisian children.

(PDF emailed within 1 workday: $29.90)

Accession: 057802744

Download citation: RISBibTeXText

PMID: 25909062

Related references

Etiology of acute diarrhea in children and adults in Tunis, Tunisia, with emphasis on diarrheagenic Escherichia coli: prevalence, phenotyping, and molecular epidemiology. American Journal of Tropical Medicine and Hygiene 77(3): 571-582, 2007

Association of diarrheagenic Escherichia coli Pathotypes with infection and diarrhea among Mexican children and association of atypical Enteropathogenic E. coli with acute diarrhea. Journal of Clinical Microbiology 47(1): 93-98, 2008

Frequency and virulence properties of diarrheagenic Escherichia coli in children with diarrhea in Gabon. American Journal of Tropical Medicine and Hygiene 69(4): 406-410, 2003

Involvement of main diarrheagenic Escherichia coli, with emphasis on enteroaggregative E. coli, in severe non-epidemic pediatric diarrhea in a high-income country. Bmc Infectious Diseases 15: 79, 2015

High Prevalence of Diarrheagenic Escherichia coli among Children with Diarrhea in Kenya. Japanese Journal of Infectious Diseases 70(1): 80-83, 2016

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil. Memorias do Instituto Oswaldo Cruz 100(4): 359-363, 2005

Diarrheagenic Escherichia coli associated with acute diarrhea in children of CumanĂ¡, Venezuela. Investigacion Clinica 51(4): 489-500, 2011

The role of diarrheagenic Escherichia coli in acute diarrhea of children in Karaj, Iran. American Journal of Gastroenterology 98(9 Supplement): S74, 2003

Prevalence and antimicrobial resistance of diarrheagenic Escherichia coli and Shigella species associated with acute diarrhea in Tehran, Iran. Canadian Journal of Infectious Diseases and Medical Microbiology 20(3): E56-E62, 2010

Prevalence and Association of Escherichia coli and Diarrheagenic Escherichia coli in Stored Foods for Young Children and Flies Caught in the Same Households in Rural Bangladesh. American Journal of Tropical Medicine and Hygiene 98(4): 1031-1038, 2018

Occurrence and antimicrobial drug susceptibility patterns of commensal and diarrheagenic Escherichia coli in fecal microbiota from children with and without acute diarrhea. Journal of Microbiology 49(1): 46-52, 2011

Bovine colostrum ameliorates diarrhea in infection with diarrheagenic Escherichia coli, shiga toxin-producing E. Coli, and E. coli expressing intimin and hemolysin. Journal of Pediatric Gastroenterology and Nutrition 29(4): 452-456, 1999

Etiological agents of diarrhea in hospitalized pediatric patients with special emphasis on diarrheagenic Escherichia coli in North India. Journal of Laboratory Physicians 11(1): 68-74, 2019

Etiology of infantile diarrhea. XVIII. Escherichia coli enteropathogen in acute diarrhea and toxicosis in small children. Revista Chilena de Pediatria 27(6): 233-41; Contd, 1956

Prevalence of diarrheagenic Escherichia coli strains detected by PCR in patients with travelers' diarrhea. Clinical Microbiology and Infection 4(12): 682-688, 2002