+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

F1 -ATP synthase α-subunit: a potential target for RNAi-mediated pest management of Locusta migratoria manilensis



F1 -ATP synthase α-subunit: a potential target for RNAi-mediated pest management of Locusta migratoria manilensis



Pest Management Science 72(7): 1433-1439



The migratory locust is one of the most destructive agricultural pests worldwide. ATP synthase (F0 F1 -ATPase) uses proton or sodium motive force to produce 90% of the cellular ATP, and the α-subunit of F1 -ATP synthase (ATP5A) is vital for F1 -ATP synthase. Here, we tested whether ATP5A could be a potential target for RNAi-mediated pest management of L. migratoria. Lm-ATP5A was cloned and characterised. Lm-ATP5A is expressed in all tissues. Injection of 100 ng of the double-stranded RNA of ATP5A (dsATP5A) knocked down the transcription of the target gene and caused mortality in 1.5-5 days. The Lm-ATP5A protein level, the oligomycin-sensitive ATP synthetic and hydrolytic activities and the ATP content were correspondingly reduced following dsATP5A injection. These findings demonstrated the essential roles of Lm-ATP5A in L. migratoria and identified it as a potential target for insect pest control. © 2015 Society of Chemical Industry.

(PDF emailed within 0-6 h: $19.90)

Accession: 057851863

Download citation: RISBibTeXText

PMID: 26558746

DOI: 10.1002/ps.4185


Related references

Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen). Insect Biochemistry and Molecular Biology 40(11): 824-833, 2011

Drought and locust plagues in China the case of Locusta migratoria manilensis Meyen Secheresse et fleau acridien en Chine le cas du criquet migrateur, Locusta migratoria manilensis Meyen. Secheresse. juin; 72: 105-108, 1996

Optimization of parental RNAi conditions for hunchback gene in Locusta migratoria manilensis. Insect Science: 1, 1-6, 2010

Role of hunchback in segment patterning of Locusta migratoria manilensis revealed by parental RNAi. Development, Growth and Differentiation 48(7): 439-445, 2006

Cytological studies on migratory locust hybrid, Locusta migratoria migratoria L. X Locusta migratoria manilensis Meyen. Acta Zool Sinica 10(1): 53-59, 1958

Phagorepellency of neem (Azadirachta indica A. Juss) oil: its potential use in the management of the migratory locust, Locusta migratoria manilensis (Meyen). Philippine Entomologist 20(2): 126-136, 2006

Rearing Locusta migratoria manilensis Locusta migratoria and migratoria on semisynthetic media. Zool Zh 45(6): 858-864, 1966

Vacuolar ATPase subunit H is essential for the survival and moulting of Locusta migratoria manilensis. Insect Molecular Biology 21(4): 405-413, 2013

Initial analysis of the hemocyanin subunit type 1 (Hc1 gene) from Locusta migratoria manilensis. Molecular Biology Reports 39(3): 3305-3310, 2012

ER type I signal peptidase subunit (LmSPC1) is essential for the survival of Locusta migratoria manilensis and affects moulting, feeding, reproduction and embryonic development. Insect Molecular Biology 23(3): 269-285, 2014

Rearing Locusta migratoria manilensis Mey and L.m. migratoria L. on semi-synthetic media. Zool. Zh, 45: pt. 6, 858-864, 1966

Evaluation of some potential target genes and methods for RNAi-mediated pest control of the corn earworm Helicoverpa zea. Pesticide Biochemistry and Physiology 149: 67-72, 2018

An outbreak of Locusta migratoria manilensis on Magesima Island. Insectarium, 242: 44-55, 1987

Purification and identification of allergens in Locusta migratoria manilensis. Chinese Journal of Applied Entomology 49(1): 244-247, 2012

Purification of glutathione S-transferase from Locusta migratoria manilensis. Kunchong Zhishi 46(3): 480-484, 2009