+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Gene expression profiling of DMU-212-induced apoptosis and anti-angiogenesis in vascular endothelial cells

Gene expression profiling of DMU-212-induced apoptosis and anti-angiogenesis in vascular endothelial cells

Pharmaceutical Biology 54(4): 660-666

trans-3,4,5,4'-Tetramethoxystilbene (DMU-212), an derivative of resveratrol, shows strong antiproliferative activities against many cancer cells. In our previous study, we demonstrated that DMU-212 possesses potent proapoptosis and antiangiogenesis effects on vascular endothelial cells (VECs), which made it a promising agent for the treatment of angiogenesis-related diseases. We studied the gene expression profile of DMU-212-treated VECs to gain further insight into the mechanisms by which DMU-212 exerts its potent pro-apoptosis and antiangiogenesis effects. The potential changes in the gene expression of VECs incubated with DMU-212 were identified and analyzed using the Affymetrix HG-U133 Plus 1.0 array. In addition, the gene expression profile was validated by quantitative real-time PCR (qRT-PCR) analysis for seven of those altered genes. DMU-212 was found to regulate a diverse range of genes, including cytokines (IL8, selectin E, MPZL2, EGR1, CCL20, ITGB8, CXCL1, VCAM1, KITLG, and AREG), transport proteins (TRPC4, SLC41A2, SLC17A5, and CREB5), metabolism (CYP1B1, CYP1A1, PDK4, CSNK1G1, MVK, TCEB3C, and CDKN3), enzymes (RAB23, SPHK1, CHSY3, PLAU, PLA2G4C, and MMP10), and genes involved in signal transduction (TMEM217, DUSP8, and SPRY4), chromosome organization (HIST1H2BH and GEM), cell migration and angiogenesis (ERRFI1, HBEGF, and NEDD9), and apoptosis (TNFSF15, TNFRSF9, CD274, BCL2L11, BIRC3, TNFAIP3, and TIFA), as well as other genes with unknown function (PGM5P2, SNORD1142, LOC151760, KRTAP5-2, C1orf110, SNORA14A, MIR31, C2CD4B, SCARNA4, C2orf66, SC4MOL, LOC644714, and LOC283392). This is the first application of microarray technique to investigate and analyze the profile of genes regulated by DMU-212 in VECs. Our results lead to an increased understanding of the signaling pathways involved in DMU-212-induced apoptosis and antiangiogenesis.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 057923645

Download citation: RISBibTeXText

PMID: 26428916

DOI: 10.3109/13880209.2015.1071414

Related references

Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion. Arthritis Research and Therapy 12(4): R131, 2010

Angiogenesis activators and inhibitors differentially regulate caveolin-1 expression and caveolae formation in vascular endothelial cells. Angiogenesis inhibitors block vascular endothelial growth factor-induced down-regulation of caveolin-1. Journal of Biological Chemistry 274(22): 15781-5, 1999

An approach to gene expression profiling of endothelial cells in angiogenesis. Molecular Biology of the Cell 10(Suppl. ): 324a, 1999

Comparative gene expression profiling of human umbilical vein endothelial cells and ocular vascular endothelial cells. British Journal of Ophthalmology 96(1): 128-132, 2012

Pigment epithelium-derived factor inhibits leptin-induced angiogenesis by suppressing vascular endothelial growth factor gene expression through anti-oxidative properties. Microvascular Research 65(3): 186-190, 2003

Bp525 inhibits vascular endothelial growth factor-induced angiogenesis and HIF-1? expression on endothelial cells. 2012

Bp5250 inhibits vascular endothelial growth factor-induced angiogenesis and HIF-1α expression on endothelial cells. Naunyn-Schmiedeberg's Archives of Pharmacology 385(1): 39-49, 2012

Gene expression profiling of microvascular endothelial cells during capillary morphogenesis in an in vitro model of angiogenesis. Zhonghua Wai Ke Za Zhi 43(1): 37-41, 2005

Angiogenesis induced by endothelial nitric oxide synthase gene through vascular endothelial growth factor expression in a rat hindlimb ischemia model. Circulation 108(18): 2250-2257, 2003

The role of the vascular endothelial growth factor/vascular endothelial growth factor receptors axis mediated angiogenesis in curcumin-loaded nanostructured lipid carriers induced human HepG2 cells apoptosis. Journal of Cancer Research and Therapeutics 11(3): 597-605, 2016

Dihydroartemisinin inhibits angiogenesis induced by multiple myeloma RPMI8226 cells under hypoxic conditions via downregulation of vascular endothelial growth factor expression and suppression of vascular endothelial growth factor secretion. Anti-Cancer Drugs 17(7): 839-848, 2006

Vascular endothelial growth factor regulates both apoptosis and angiogenesis of choriocapillaris endothelial cells. Microvascular Research 59(2): 286-289, 2000

Expression profiling of ETS and MMP factors in VEGF-activated endothelial cells: role of MMP-10 in VEGF-induced angiogenesis. Journal of Cellular Physiology 224(3): 734-742, 2010

Microarray gene expression profiling reveals interference of the anti-oxidants N-acetyl-cysteine and -epigallocatechin-3-gallate with endothelial cell apoptosis, migration and response to inflammation. Cancer Epidemiology Biomarkers & Prevention 12(11 Part 2): 1348s, 2003

Toona sinensis (leaf extracts) inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis in vascular endothelial cells. Journal of Ethnopharmacology 134(1): 111-121, 2011